Proteomics

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Comprehensive proteomics for Discovery vaccine candidates against Streptococcus equi Infections

ABSTRACT: Streptococcus equi subspecies equi (S. equi) is a major pathogen which cause strangles, a highly contagious respiratory infection, in horses and other equines.In this study, we discovered potential vaccine candidates using comprehensive proteomics and reverse vaccinology. As the initial step, we divided proteome of S. equi ATCC 39506 into whole cell lysate, secretory proteome, membrane proteome and extracellular vesicle and then, comparative proteomic analysis was performed to characterize the functional features of the proteome. Especially, extracellular vesicle of S. equi was evaluated at the first time. Total 114 potential vaccine candidates (PVCs) were selected using reverse vaccinology and knowledge based annotations. Comprehensive proteomic analysis confirmed that 60 PVCs were identified in S. equi ATCC 39506. Particularly, 32 PVCs were enriched in the EV proteome, suggesting that this cellular fraction may serve as vaccine.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Streptococcus Equi Subsp. Equi 4047

TISSUE(S): Cell Culture

SUBMITTER: Hayoung Lee  

LAB HEAD: Seung Il Kim

PROVIDER: PXD025152 | Pride | 2021-08-06

REPOSITORIES: Pride

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Publications

Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection.

Lee Hayoung H   Yun Sung Ho SH   Hyon Ju-Yong JY   Lee Sang-Yeop SY   Yi Yoon-Sun YS   Choi Chi-Won CW   Jun Sangmi S   Park Edmond Changkyun EC   Kim Seung Il SI  

Veterinary microbiology 20210625

Streptococcus equi subspecies equi is a pathogenic bacterium that causes strangles, a highly contagious respiratory infection in horses and other equines. The limitations of current vaccines against S. equi infection warrants the development of an affordable, safe, and effective vaccine. Because gram-positive extracellular vesicles (EVs) transport various immunogenic antigens, they are attractive vaccine candidates. Here, we purified the EVs of S. equi ATCC 39506 and evaluated them as a vaccine  ...[more]

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