Dataset Information

Investigation on the quantitative proteomic responses of bloom-forming Cyanobacteria Microcystis aeruginosa to phosphorus depletion and starvation by the method of isobaric tags for relative and absolute quantitation (iTRAQ)

ABSTRACT: Microcystis aeruginosa cells were treated with phosphorus repletion, depletion and starvation. Isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was employed to explore to the effects of phosphorus limitation on Microcystis aeruginosa cells at the protein level. This investigation would contribute to the understanding of global cellular responses of Microcystis to phosphorus limitation and provide theoretical basis for deciding whether it is an effective way to control Microcystis blooms by phosphorus reduction.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Microcystis Aeruginosa

TISSUE(S): Photosynthetic Cell, Cell Culture

SUBMITTER: Nian Wei

LAB HEAD: Lirong Song

PROVIDER: PXD025188 | Pride | 2021-06-29

REPOSITORIES: Pride

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Quantitative Proteomic and Microcystin Production Response of Microcystis aeruginosa to Phosphorus Depletion.

Wei Nian N Song Lirong L Gan Nanqin N

Microorganisms 20210531 6

Microcystis blooms are the most widely distributed and frequently occurring cyanobacterial blooms in freshwater. Reducing phosphorus is suggested to be effective in mitigating cyanobacterial blooms, while the underlying molecular mechanisms are yet to be elucidated. In the present study, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics was employed to study the effects of phosphorus depletion on Microcystis aeruginosa FACHB-905. The product ...[more]

PMID: 34072711

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Project description:Proteins secreted by marine cyanobacterium Synechococcus under phosphorus stress is largely uncharacterized. This dataset characterizes the exoproteins for both an open ocean (WH8102) and coastal (WH5701) Synechococcus strain and were collected as part of the study "Dissolved organic phosphorus bond-class utilization by Synechococcus". Study Abstract: Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP’s importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5’-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5’-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5’-triphosphate, and then adenosine 5’-monophosphate. The strains’ exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5’-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus’ exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.

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Dataset Information

Investigation on the quantitative proteomic responses of bloom-forming Cyanobacteria Microcystis aeruginosa to phosphorus depletion and starvation by the method of isobaric tags for relative and absolute quantitation (iTRAQ)

Dataset's files

Publications

Quantitative Proteomic and Microcystin Production Response of <i>Microcystis aeruginosa</i> to Phosphorus Depletion.

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