Proteomics

Dataset Information

0

27-plex Tandem Mass Tag Mass Spectrometry for Profiling Brain Proteome in Alzheimer's Disease


ABSTRACT: Systematically optimized the protocol for the newly developed 16-plex TMT Reported a novel 27-plex method to enhance the multiplexity for large-scale proteomic profiling, by combining two sets of distinct isobaric tags (11-plex and 16-plex TMT) based on their disparate mass shifts on modified peptides.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Cerebral Cortex

DISEASE(S): Alzheimer's Disease

SUBMITTER: Zhen Wang  

LAB HEAD: Junmin Peng

PROVIDER: PXD025261 | Pride | 2021-04-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
27plex_TMT11_f1.pepXML Pepxml
27plex_TMT11_f10.pepXML Pepxml
27plex_TMT11_f11.pepXML Pepxml
27plex_TMT11_f12.pepXML Pepxml
27plex_TMT11_f13.pepXML Pepxml
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Publications

27-Plex Tandem Mass Tag Mass Spectrometry for Profiling Brain Proteome in Alzheimer's Disease.

Wang Zhen Z   Yu Kaiwen K   Tan Haiyan H   Wu Zhiping Z   Cho Ji-Hoon JH   Han Xian X   Sun Huan H   Beach Thomas G TG   Peng Junmin J  

Analytical chemistry 20200507 10


Multiplexed isobaric labeling methods, such as tandem mass tags (TMT), remarkably improve the throughput of quantitative mass spectrometry. Here, we present a 27-plex TMT method coupled with two-dimensional liquid chromatography (LC/LC) for extensive peptide fractionation and high-resolution tandem mass spectrometry (MS/MS) for peptide quantification and then apply the method to profile the complex human brain proteome of Alzheimer's disease (AD). The 27-plex method combines multiplexed capaciti  ...[more]

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