Proteomics

Dataset Information

0

Identification of ESAG4-interacting proteins in Trypanosoma brucei bloodstream forms

ABSTRACT: How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. Flagellar cAMP signaling is implicated in interaction of the unicellular parasite Trypanosoma brucei with its hosts. The second messenger is produced by a large and atypical family of receptor-type adenylate cyclases (ACs) with unknown regulation mechanism. In order to identify trypanosome AC interacting proteins, ESAG4, an AC highly abundant in the parasite’s bloodstream stage, was fused to GFP and purified via GFP trap pull down followed by mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trypanosoma Brucei Brucei

SUBMITTER: Ignasi Forne  

LAB HEAD: Boshart, Michael

PROVIDER: PXD025412 | Pride | 2022-08-31

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Groups.txt Txt
Ref3449_MB_S01_r1_20201006.raw Raw
Ref3449_MB_S01_r2_20201006.raw Raw
Ref3449_MB_S01_r3_20201006.raw Raw
Ref3449_MB_S02_r1_20201006.raw Raw
Items per page:
1 - 5 of 26
altmetric image

Publications

Signaling from ciliary microdomains controls developmental processes in metazoans. Trypanosome transmission requires development and migration in the tsetse vector alimentary tract. Flagellar cAMP signaling has been linked to parasite social motility (SoMo) in vitro, yet uncovering control of directed migration in fly organs is challenging. Here we show that the composition of an adenylate cyclase (AC) complex in the flagellar tip microdomain is essential for tsetse salivary gland (SG) colonizat  ...[more]

Similar Datasets

Proteomics Identification of CARP3-interacting proteins in Trypanosoma brucei bloodstream forms and procyclic forms
2022-08-31 | PXD025398 | Pride
Proteomics Quantitative proteomics upon CARP3 knock down in Trypanosoma brucei bloodstream forms
2022-08-31 | PXD025401 | Pride
Proteomics Identification of novel Coronin 1A interacting proteins
2017-06-29 | PXD004635 | Pride
Proteomics SAMD1 suppresses epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma.
2024-07-12 | PXD044104 | Pride
Proteomics CTCF loss has limited effects on genome architecture in Drosophila despite critical regulatory functions.
2021-01-18 | PXD019487 | Pride
Proteomics IRF2BP2 counteracts the ATF7/JDP2 heterodimer to prevent inflammatory overactivation in AML cells
2024-05-16 | PXD041817 | Pride
Proteomics Identification of FLS2 interacting proteins by co-immunoprecipitation and shot gun proteomics
2016-01-26 | PXD003189 | Pride
Proteomics Peptide-mediated inhibition of the transcriptional regulator Elongin BC induces apoptosis in cancer cells
2023-06-27 | PXD038414 | Pride
Proteomics Identification of ubiquitination targets of UHRF1
2023-01-30 | PXD034335 | Pride
Proteomics The histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA-binding winged helix domainThe histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA-binding winged helix domain
2023-01-14 | PXD034833 | Pride