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Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ

ABSTRACT: Differences between species promote stable coexistence in a resource-limited environment. These differences can result from interspecies competition leading to character shifts, a process referred to as character displacement. While character displacement is often interpreted as a consequence of genetically fixed trait differences between species, it can also be mediated by phenotypic plasticity in response to the presence of another species. Here, we test whether phenotypic plasticity leads to a shift in proteome allocation during co-occurrence of two bacterial species from the abundant, leaf-colonizing families Sphingomonadaceae and Rhizobiaceae in their natural habitat. Upon mono-colonizing of the phyllosphere, both species exhibit specific and shared protein functions indicating a niche overlap. During co-colonization, quantitative differences in the protein repertoire of both bacterial populations occur as a result of bacterial coexistence in planta. Specifically, the Sphingomonas strain produces enzymes for the metabolization of xylan, while the Rhizobium strain reprograms its metabolism to beta-oxidation of fatty acids fueled via the glyoxylate cycle and adapts its biotin acquisition. We demonstrate the conditional relevance of cross-species facilitation by mutagenesis leading to loss of fitness in competition in planta. Our results show that dynamic character displacement and niche facilitation mediated by phenotypic plasticity can contribute to species coexistence.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Rhizobium Sp. Leaf68 Sphingomonas Sp. Leaf257

TISSUE(S): True Leaf

SUBMITTER: Lucas Hemmerle

LAB HEAD: Julia A. Vorholt

PROVIDER: PXD026619 | Pride | 2022-05-26

REPOSITORIES: Pride

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			Action	DRS
	20190309_003_S193461_LH021_L257_plant_2.raw	Raw
	20190309_004_S193451_LH021_L68_plant_4.raw	Raw
	20190309_005_S193463_LH021_L257_plant_4.raw	Raw
	20190309_006_S193456_LH021_L257_plate_1.raw	Raw
	20190309_008_S193458_LH021_L257_plate_3.raw	Raw

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Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ

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