Dataset Information

CD68+ region of rat scar tissue by AutoSTOMP

ABSTRACT: To test the ability of the Automated Spatially Targeted Optical Micro Proteomics (AutoSTOMP) protocol to selectively biotinylate structures of interest within tissue sections we first examined a rat myocardial infarction model. In this model, trauma caused by ligation and infiltrating immune cells causes fibroblast activation and deposition of scar tissue that ultimately impairs cardiac function. Macrophages are thought to play a role in inflammatory regulation and damaged cell turnover in the tissue. We decide to grab the proteome of the macrophage rich regions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart

SUBMITTER: BOCHENG YIN

LAB HEAD: Sarah E. Ewald

PROVIDER: PXD026818 | Pride | 2021-08-10

REPOSITORIES: Pride

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Automated Spatially Targeted Optical Microproteomics Investigates Inflammatory Lesions <i>In Situ</i>.

Yin Bocheng B Caggiano Laura R LR Li Rung-Chi RC McGowan Emily E Holmes Jeffrey W JW Ewald Sarah E SE

Journal of proteome research 20210826 9

Tissue microenvironment properties like blood flow, extracellular matrix, or proximity to immune-infiltrate are important regulators of cell biology. However, methods to study regional protein expression in the native tissue environment are limited. To address this need, we developed a novel approach to visualize, purify, and measure proteins <i>in situ</i> using automated spatially targeted optical microproteomics (AutoSTOMP). Here, we report custom codes to specify regions of heterogeneity in ...[more]

PMID: 34436902

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