Proteomics

Dataset Information

0

FPB - 2021 - Azri et al - Physiological and proteomic analyses of Tunisian local grapevine cultivar Razegui in response to drought stress.


ABSTRACT: Cuttings from Razegui cultivar were cultivated in sand for 2 months according to the method we have described previously (Azri et al. 2020). Then grapevine plantlets were further grown in pots containing sand/peat mixture (2/1, v/v), in a controlled greenhouse (16 h light/8 h dark at PAR 300 μmol m-2 s-1 photoperiod, 24 °C day/20 °C night temperature and 65-75% relative humidity). They were watered daily with tap water and supplemented once a week with 100 mL of Gibeaut’s nutrient solution (Gibeaut et al. 1997). Drought stress was applied according to our previously described procedure (Azri et al. 2020): plants with 10-15 leaves were either irrigated daily with water (control) or non-irrigated at all (water deficit). After 4 time points (day 0, 4, 8 and 16) of water deficit, the 4th fully-developed leaf was harvested and used for physiological analyses. Three independent biological experiments were performed consisting each in 2 treatments (control and water deficit) and 3 plants per time point and treatment. Proteomic analysis was performed on the 5th fully developed leaves at the time point 16 days of drought treatment. Irrigated and non-irrigated leaves were harvested immediately in liquid nitrogen and stored at - 80 °C until analysis. Three biological replicates were carried out for each treatment.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Pascal COSETTE  

LAB HEAD: Ahmed Mliki

PROVIDER: PXD027073 | Pride | 2021-08-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AZRI_1.msf Msf
AZRI_10.msf Msf
AZRI_11.msf Msf
AZRI_12.msf Msf
AZRI_13.msf Msf
Items per page:
1 - 5 of 98

Similar Datasets

2011-08-25 | E-GEOD-31677 | biostudies-arrayexpress
2007-05-20 | GSE4971 | GEO
2012-08-01 | GSE33574 | GEO
2017-12-11 | PXD001661 | Pride
2011-08-26 | GSE31677 | GEO
2012-04-27 | GSE37608 | GEO
2011-01-15 | E-GEOD-17230 | biostudies-arrayexpress
2012-04-26 | E-GEOD-37608 | biostudies-arrayexpress
2011-01-15 | GSE17230 | GEO
2011-01-15 | GSE17226 | GEO