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Hyperosmolality in CHO Culture: Effects on Proteome

ABSTRACT: Chinese hamster ovary cells (CHO) were exposed to highly concentrated feed solution during a fed-batch cultivation which causes an unphysiological osmolality increase (>300 mOsm/kg) affecting cell physiology, morphology and proteome. To get a deeper insight into underlying molecular mechanisms involved in cellular hyperosmolality response, we performed a comparative quantitative label-free proteome study of hyperosmolality-exposed vs. control CHO cells by nanoLC-ESI-MS measurement approach (orbitrap-MS). Our observations correlate well with the data from recent CHO-based fluxome and transcriptome studies and expose hitherto unknown targets involved in response to hyperosmotic pressure in mammalian cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Suspension Culture, Permanent Cell Line Cell

SUBMITTER: Louise Schelletter

LAB HEAD: Prof. Thomas Noll

PROVIDER: PXD027443 | Pride | 2022-05-20

REPOSITORIES: Pride

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Publications

Hyperosmolality in CHO cell culture: effects on the proteome.

Romanova Nadiya N Schelletter Louise L Hoffrogge Raimund R Noll Thomas T

Applied microbiology and biotechnology 20220321 7

Chinese hamster ovary (CHO) cells are the most commonly used host cell lines for therapeutic protein production. Exposure of these cells to highly concentrated feed solution during fed-batch cultivation can lead to a non-physiological increase in osmolality (> 300 mOsm/kg) that affects cell physiology, morphology, and proteome. As addressed in previous studies (and indeed, as recently addressed in our research), hyperosmolalities of up to 545 mOsm/kg force cells to abort proliferation and gradua ...[more]

PMID: 35312825

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