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Venomics of the desert black cobra Walterinnesia aegyptia from Sinai Peninsula, Egypt

ABSTRACT: Combined transcriptomics-assisted bottom-up and top-down MS characterization of the venom proteome of the desert black cobra Walterinnesia aegyptia from Sinai Peninsula, Egypt.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Serpentes Sp. Wls21042014

SUBMITTER: Davinia Pla

LAB HEAD: Juan J Calvete

PROVIDER: PXD027495 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani.

Calvete Juan J JJ Pla Davinia D Els Johannes J Carranza Salvador S Damm Maik M Hempel Benjamin-Florian BF John Elisa B O EBO Petras Daniel D Heiss Paul P Nalbantsoy Ayse A Göçmen Bayram B Süssmuth Roderich D RD Calderón-Celis Francisco F Nosti Alicia Jiménez AJ Encinar Jorge Ruiz JR

Journal of proteome research 20211004 11

We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus Walterinnesia, Walterinnesia aegyptia and Walterinnesia morgani. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for ...[more]

PMID: 34606723

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