Dataset Information

Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system

ABSTRACT: Long-distance regeneration of the central nervous system (CNS) has been achieved from the eye to the brain through activation of neuronal molecular pathways or pharmacological approaches. Characterizing the mature neuronal environment is essential to understand the adult axonal guidance in order to complete the circuit reconstruction. To this end, we used mass spectrometry (MS)-based quantitative proteomics to characterize the proteomes of major nuclei of the adult visual system: suprachiasmatic nucleus (SCN), ventral and dorsal lateral geniculate nucleus (vLGN, dLGN) and superior colliculus (SCol)), as well as the optic chiasm. These analyses revealed the presence of guidance molecules and guidance-associated factors in the adult visual targets. Moreover, by performing bilateral optic nerve crush, we showed that the expression of specific proteins was significantly modulated by the injury in the visual targets, even in the ones most distal to the lesion site. On another hand, we found that the expression of guidance molecules was not modified upon injury and that regenerative axons are able to respond to guidance cues. Together, our results provide an extensive characterization of the molecular environment in intact and injured conditions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Hesse Anne-Marie

LAB HEAD: Yohann Coute

PROVIDER: PXD029325 | Pride | 2022-10-20

REPOSITORIES: Pride

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Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.

Vilallongue Noemie N Schaeffer Julia J Hesse Anne-Marie AM Delpech Céline C Blot Béatrice B Paccard Antoine A Plissonnier Elise E Excoffier Blandine B Couté Yohann Y Belin Stephane S Nawabi Homaira H

Nature communications 20221013 1

In the injured adult central nervous system (CNS), activation of pro-growth molecular pathways in neurons leads to long-distance regeneration. However, most regenerative fibers display guidance defects, which prevent reinnervation and functional recovery. Therefore, the molecular characterization of the proper target regions of regenerative axons is essential to uncover the modalities of adult reinnervation. In this study, we use mass spectrometry (MS)-based quantitative proteomics to address th ...[more]

PMID: 36229455

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			Action	DRS
	2021_NAWABIBELIn_nocrush.mzid.gz	Mzid
	HF1_008420.mgf	Mgf
	HF1_008420.raw	Raw
	HF1_008424.mgf	Mgf
	HF1_008424.raw	Raw