Dataset Information

Proteome profiling of Campylobacter jejuni 81-176 at 37°C and 42°C by label-free mass spectrometry

ABSTRACT: Background: The main natural reservoir for Campylobacter jejuni is the intestinal tract of birds, especially in the context of human nutrition chickens and turkeys play the main role. There C. jejuni multiplies optimally at 42°C, the avian body temperature, while after infection of humans by peroral intake in the human intestinal tract only 37°C prevail. Methods: Proteome profiling by label-free mass spectrometry (SWATH-MS) was used to examine the cellular processes which enable C. jejuni strain 81-176 to adapt to 37°C in comparison to 42°C. In total, four states were compared with each other: incubation for 12 h at 37°C, for 24 h at 37°C, for 12 h at 42°C and 24 h at 42°C. Results: It was shown that the proteome profile changes not only according to the different incubation temperature but also with the length of the incubation period, i.e. significant differences in protein expression were already evident when comparing 12 h and 24 h. Altogether, the expression change of 957 proteins could be recorded. Out of these 171 proteins were considered to be significantly differentially expressed, that means these proteins showed at least a 1.5-fold change in either direction (i.e log2(FC)≥0.585 or log2(FC)≤-0.585) and an FDR-adjusted p-value less than 0.05. The significantly differentially expressed proteins could be arranged in 5 different clusters and several functional groups involving e.g. motility & chemotaxis, metabolism, stress response, rDNA translation and DNA replication.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Campylobacter Jejuni

DISEASE(S): Gastroenteritis

SUBMITTER: Christof Lenz

LAB HEAD: Christof Lenz

PROVIDER: PXD029494 | Pride | 2024-06-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20160718_CAMJJ_Proteome_wCRAP.fasta	Fasta
	Masanta_et_al_Sample_List_DDA_and_DIA.xlsx	Xlsx
	W_Masanta_060416_020816_SWATH_10a_1.wiff	Wiff
	W_Masanta_060416_020816_SWATH_10a_1.wiff.scan	Wiff
	W_Masanta_060416_020816_SWATH_10a_2.wiff	Wiff

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Publications

Proteome profiling of Campylobacter jejuni 81-176 at 37 °C and 42 °C by label-free mass spectrometry.

Dreyer Annika A Masanta Wycliffe O WO Lugert Raimond R Bohne Wolfgang W Groß Uwe U Leha Andreas A Dakna Mohammed M Lenz Christof C Zautner Andreas E AE

BMC microbiology 20240531 1

<h4>Background</h4>The main natural reservoir for Campylobacter jejuni is the avian intestinal tract. There, C. jejuni multiplies optimally at 42 °C - the avian body temperature. After infecting humans through oral intake, the bacterium encounters the lower temperature of 37 °C in the human intestinal tract. Proteome profiling by label-free mass spectrometry (DIA-MS) was performed to examine the processes which enable C. jejuni 81-176 to thrive at 37 °C in comparison to 42 °C. In total, four sta ...[more]

PMID: 38822261

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