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Quantitative phosphoproteomics study for assessing rodent thyroid toxicity

ABSTRACT: Here, we aimed to apply a TMT based quantitative phosphoproteomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Phosphoproteomics were applied to liver.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Liver

SUBMITTER: Zhipeng Wang

LAB HEAD: Kristin Schubert

PROVIDER: PXD030254 | Pride | 2024-10-25

REPOSITORIES: Pride

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			Action	DRS
	Rattus-norvegicus_uniprot-proteome_reference_29944_20200803_ZW.fasta	Fasta
	XomeTox_L_tmtpp_ft_set1_f1.raw	Raw
	XomeTox_L_tmtpp_ft_set1_f2.raw	Raw
	XomeTox_L_tmtpp_ft_set2_f1.raw	Raw
	XomeTox_L_tmtpp_ft_set2_f2.raw	Raw

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