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High-throughput identification of RNA localization elements reveals a regulatory role for A/G rich sequences

ABSTRACT: This experiment aims to identify the RNA-binding proteome that is pulled-down from a cellular lysate with biotinylated RNA probes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Andreas Moor

LAB HEAD: Andreas Moor

PROVIDER: PXD030720 | Pride | 2022-09-21

REPOSITORIES: Pride

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			Action	DRS
	1840994.zip	Other
	1864460.zip	Other
	20210326_C24489_002_S290491_CAD_FLUC_1_Control.raw	Raw
	20210326_C24489_004_S290492_CAD_NET_1_Group_1.raw	Raw
	20210326_C24489_006_S290493_CAD_TRAK_1_Group_2.raw	Raw

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Publications

High-throughput identification of RNA localization elements in neuronal cells.

Arora Ankita A Castro-Gutierrez Roberto R Moffatt Charlie C Eletto Davide D Becker Raquel R Brown Maya M Moor Andreas E AE Russ Holger A HA Taliaferro J Matthew JM

Nucleic acids research 20221001 18

Hundreds of RNAs are enriched in the projections of neuronal cells. For the vast majority of them, though, the sequence elements that regulate their localization are unknown. To identify RNA elements capable of directing transcripts to neurites, we deployed a massively parallel reporter assay that tested the localization regulatory ability of thousands of sequence fragments drawn from endogenous mouse 3' UTRs. We identified peaks of regulatory activity within several 3' UTRs and found that seque ...[more]

PMID: 36107770

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A massively parallel reporter assay reveals novel features and players of RNA localization in neurons

Project description:This experiment aims to identify the RNA-binding proteome that is pulled-down from a cellular lysate with biotinylated RNA probes.

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Early cleavage of preimplantation embryos is regulated by tRNAGln-TTG-derived small RNAs present in mature spermatozoa

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