Proteomics

Dataset Information

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Pytheas S. cerevisiae tRNA sample analysis

ABSTRACT: Mass spectrometry remains an important method for analysis of modified nucleosides ubiquitously present in cellular RNAs, in particular for ribosomal and transfer RNAs that play crucial roles in mRNA translation and decoding. Furthermore, modifications have effect on the lifetimes of nucleic acids in plasma and cells and are consequently incorporated into RNA therapeutics. To provide an analytical tool for sequence characterization of modified RNAs, we developed Pytheas, an open-source software package for automated analysis of tandem MS data for RNA. This dataset contains the analysis of a mixture of S. cerevisiae tRNAs, decorated with a variety of complex RNA modifications.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Luigi D'Ascenzo  

LAB HEAD: Jamer R. Williamson

PROVIDER: PXD030844 | Pride | 2022-05-04

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Digest_MS2_tRNA_yeast.txt Txt
match_output_tRNA_yeast_Roche_250pmoles_7.5uL.txt Txt
modfile_tRNA_yeast_noY Other
nts_alphabet_light_tRNAyeast.xlsx Xlsx
sacCer3-mature-tRNAs.fa Other
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Publications

Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry.

D'Ascenzo Luigi L   Popova Anna M AM   Abernathy Scott S   Sheng Kai K   Limbach Patrick A PA   Williamson James R JR  

Nature communications 20220503 1

Mass spectrometry is an important method for analysis of modified nucleosides ubiquitously present in cellular RNAs, in particular for ribosomal and transfer RNAs that play crucial roles in mRNA translation and decoding. Furthermore, modifications have effect on the lifetimes of nucleic acids in plasma and cells and are consequently incorporated into RNA therapeutics. To provide an analytical tool for sequence characterization of modified RNAs, we developed Pytheas, an open-source software packa  ...[more]

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