Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of contact allergens on THP-1 cells – LFQ- and SILAC-based untargeted proteomics

ABSTRACT: In this study, the effects of the contact allergens BQ, DNCB, NBB, Ni, and PPD were investigated in THP-1 cells. For this purpose, LFQ- and SILAC-based untargeted proteomics were applied.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Isabel Karkossa

LAB HEAD: Kristin Schubert

PROVIDER: PXD031017 | Pride | 2023-09-13

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	10A.raw	Raw
	10B.raw	Raw
	10C.raw	Raw
	10D.raw	Raw
	10E.raw	Raw

Items per page:

1 - 5 of 81

Publications

A comparative proteomics analysis of four contact allergens in THP-1 cells shows distinct alterations in key metabolic pathways.

Höper Tessa T Karkossa Isabel I Dumit Verónica I VI von Bergen Martin M Schubert Kristin K Haase Andrea A

Toxicology and applied pharmacology 20230803

Allergic contact dermatitis (ACD) is the predominant form of immunotoxicity in humans. The sensitizing potential of chemicals can be assessed in vitro. However, a better mechanistic understanding could improve the current OECD-validated test battery. The aim of this study was to get insights into toxicity mechanisms of four contact allergens, p-benzoquinone (BQ), 2,4-dinitrochlorobenzene (DNCB), p-nitrobenzyl bromide (NBB) and NiSO<sub>4</sub>, by analyzing differential proteome alterations in T ...[more]

PMID: 37541627

Similar Datasets

Integrative analysis of miRNA/mRNA expression patterns in allergic contact dermatitis

Project description:Allergic and irritant contact dermatitis induces different immunological cascades, involving a plethora of immune cells as well as keratinocytes. This highly interwoven network is regulated by miRNAs. However, which miRNAs are involved during allergic- and irritant-induced contact dermatitis is not well investigated. Therefore, we analyzed miRNA and mRNA expression data of the same sample from positive patch test reactions from 27 patients topically exposed to allergens (nickel (Ni), epoxy resin (EP) and methylchloroisothiazolinone (CM); each n=5), irritants (sodium lauryl sulfate (SL, n=9) and nonanoic acid (NO, n=5)), as well as healthy skin (n=5).

2020-07-01 | E-MTAB-9026 | biostudies-arrayexpress

Machine learning driven biomarker discovery for the discrimination between allergic and irritant contact dermatitis

Project description:Allergic and irritant contact dermatitis induces different immunological cascades, involving a plethora of immune cells as well as keratinocytes. 96 patients were investigated using mRNA microarray experiments, of which 88 passed our QC criteria. Patients were topically exposed to allergens (nickel (Ni), epoxy resin (EP) and methylchloroisothiazolinone (CM)), irritants (sodium lauryl sulfate (SL) and nonanoic acid (NO)) for 48 hours or were left untreated.

2020-09-01 | E-MTAB-9501 | biostudies-arrayexpress

Integrative analysis of miRNA/mRNA expression patterns in allergic contact dermatitis

2020-07-01 | E-MTAB-9051 | biostudies-arrayexpress

Large-scale proteome characterization of the cassava root

Project description:Cassava is the most important root crop in the tropics but rapid post-harvest physiological root deterioration (PPD) is a major constraint to commercial cassava production. We used label-free quantitative proteomics to generate an extensive cassava root and PPD proteome. Over 2400 unique proteins were identified in the cassava root and nearly 300 proteins showed significant abundance regulation during PPD. A candidate gene for reducing PPD was identified from the regulated proteins with enzymatic assays and afterwards verified with a transgene approach. This demonstrates the relevance of proteomics approach for crop improvements.

2014-06-03 | PXD000587 | Pride

Qualitative PTM characterization of raw and roasted peanut allergens from aqueous extracts by HRMS

Project description:Project is related to immunoproteomic profiling of soluble fractions of raw and roasted peanut. It is still unknown which peanut extract is more allergenic, the raw or roasted version and part of the answer could be in revealing differences between PTM profiles of the raw and roasted peanut.

2023-03-11 | PXD033166 | Pride

Nickel-induced contact dermatitis induce changes in human skin transcriptome over 96 hours

Project description:We aimed to characterize transcriptomic changes over time (baseline, 2 hours, 48 hours and 96 hours) after nickel-induced contact dermatitis in 6 human probands. As a contrast, 7 patients with SLS-induced irritant contact dermatitis were included. Therefore, the patch tests were applied onto the back of the individuals and punch biopsies were taken at the respective time points. Only skin with clear reactions was further used for transcriptomic analysis. Using gene set enrichment analysis and leukocyte deconvolution algorithm, we were able to identify important immune cell subsets and related functions within the human skin.

2020-05-01 | E-MTAB-8945 | biostudies-arrayexpress

Transcription profiling by array of mouse BALBc strain mesenteric lymph nodes after response to common allergens.

Project description:In this study, MLN transcriptome profiling of female BALB/c mice was conducted in order to explore the molecular mechanism(s) of antigen sensitization using three of the most common food allergens; BLG, OVA and PNA. Analysis of cDNA microarray data revealed a complex network of genes that is modulated during BLG, OVA or PNA sensitization. Results from this study implicate the role of differentially expressed genes in the pathogenesis of food allergy. Results from this study will also help better understanding the molecular mechanism of food allergy in animal models and may help in developing new drug target for the therapy of food allergy. This study is likely to add a new dimension in the development of biomarker genes for the determination of potential allergenicity of genetically modified (GM) foods.

2011-05-11 | E-MEXP-2676 | biostudies-arrayexpress

Atopy and Sensitization to Allergens Known to Cause Systemic Contact Dermatitis.

Project description:BACKGROUND:Atopic dermatitis (AD) associated with respiratory atopy may represent a form of systemic contact dermatitis (SCD), whereby AD flares after ingestion or inhalation of allergens. OBJECTIVE:The aim of the study was to compare the prevalence of positive patch tests to allergens known to cause SCD in AD patients with and without respiratory atopy. METHODS:This is a retrospective study of patients with AD patch tested to 23 allergens known to cause SCD. Positive patch tests were compared between AD patients with and without respiratory atopy, stratified by age and wet or dry work occupation. CONCLUSIONS:Children and adolescents, but not adults, with AD and respiratory atopy were more likely than age-matched AD patients without respiratory atopy to have positive patch tests to these allergens (odds ratio, 2.33; 95% confidence interval, 1.13-4.79). Moreover, AD patients with respiratory atopy and engaging in wet work, but not dry work, occupations were more likely than AD patients without respiratory atopy to have positive patch tests to allergens known to cause SCD (odds ratio, 1.47; 95% confidence interval, 1.05-2.06). Thus, respiratory atopy and wet work are associated with sensitization to allergens known to cause SCD in patients with AD, and patch testing may be valuable in identifying systemic triggers of dermatitis in these patients.

| S-EPMC6334658 | biostudies-literature

Proteomic analysis of purified protein derivative of mycobacterium tuberculosis

Project description:Proteomic analysis of Connaught Tuberculin 68 (PPD-CT68), a tuberculin preparation generated froM. tuberculosis, was carried out in this study. PPD-CT68 is the protein component of a commercially available tuberculin preparation, Tubersol, which is used for tuberculin skin testing. Using a high resolution LTQ-Orbitrap Velos mass spectrometer, we identified 265 different proteins. The identified proteins were compared with those identified from PPD M. bovis, PPD M. avium and PPD-S2 from previous mass spectrometry-based studies. In all, 142 proteins were fo PPDs (i.e. proteins in either PPD-CT68 or PPD-S2), 37 proteins were found to be shared with M. avium PPD and 80 were shared with M. bovis PPD. Alignment of PPD-CT68 proteins with proteins encoded by 24 lung infecting bacteria revealed a number of similar proteins (206 bacterial proteins shared epitopes with 47 PPD-CT68 proteins), which could potentially be involved in causing cross-reactivity

2014-01-23 | PXD000377 | Pride