Dataset Information

Interactomes of GluA1 and GluA3 subunit containing AMPARs reveal distinct protein composition

ABSTRACT: The AMPA glutamate receptor (AMPAR) is the major type of synaptic excitatory ionotropic receptor in the brain. The most abundant AMPAR subtypes in the hippocampus are GluA1/2 and GluA2/3 heterotetramers. Each subtype contributes differentially to mechanisms of synaptic plasticity, which may be in part caused by how these receptors are regulated by specific associated proteins. A broad range of AMPAR interacting proteins have been identified and several were shown to affect biogenesis, AMPAR trafficking, and channel properties, alone or in distinct assemblies, and several revealed preferred binding to specific AMPAR subunits. To date, a systematic separate interactome analysis of the major GluA1/2 and GluA2/3 AMPAR subtypes is lacking. To reveal interactors belonging to specific AMPAR sub-complexes, we performed both quantitative and interaction proteomics on hippocampi of wildtype and GluA1- or GluA3 knock-out mice. Whereas GluA1/2 receptors co-purified TARP-γ8, PRRT1 and CNIH2 with highest abundances, GluA2/3 receptors revealed strongest co-purification of CNIH2, TARP-γ2, and OLFM1. Further analysis revealed that TARP-γ8-PRRT1 can interact directly, and co-assemble into an AMPAR subcomplex especially near the synapse.

INSTRUMENT(S): TripleTOF 5600, LTQ Orbitrap Discovery

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Sophie van der Spek

LAB HEAD: Ka Wan Li

PROVIDER: PXD031603 | Pride | 2022-12-12

REPOSITORIES: Pride

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Expression and Interaction Proteomics of GluA1- and GluA3-Subunit-Containing AMPARs Reveal Distinct Protein Composition.

van der Spek Sophie J F SJF Pandya Nikhil J NJ Koopmans Frank F Paliukhovich Iryna I van der Schors Roel C RC Otten Mylene M Smit August B AB Li Ka Wan KW

Cells 20221117 22

The AMPA glutamate receptor (AMPAR) is the major type of synaptic excitatory ionotropic receptor in the brain. AMPARs have four different subunits, GluA1-4 (each encoded by different genes, Gria1, Gria2, Gria3 and Gria4), that can form distinct tetrameric assemblies. The most abundant AMPAR subtypes in the hippocampus are GluA1/2 and GluA2/3 heterotetramers. Each subtype contributes differentially to mechanisms of synaptic plasticity, which may be in part caused by ho ...[more]

PMID: 36429079

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	1_A1KO_GluA1_1.RAW	Raw
	1_A1KO_GluA1_2.RAW	Raw
	1_A1KO_GluA1_3.RAW	Raw
	1_A1KO_GluA23_1.RAW	Raw
	1_A1KO_GluA23_2.RAW	Raw