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TMEM260 is a protein-specific O-mannosyltransferase

ABSTRACT: Mutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome (SHDRA), but the function of the encoded protein remains unknown. We report that TMEM260 is an ER-located protein O-mannosyltransferase that selectively glycosylates defined extracellular immunoglobulin, plexin, transcription factor (IPT) domains of the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors. We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knock out in cells results in receptor maturation defects and abnormal growth of 3D cell models. Thus, our study identifies a new, receptor-specific O-mannosylation pathway that serves critical functions during e.g. epithelial morphogenesis.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Adnan Halim

LAB HEAD: Adnan Halim

PROVIDER: PXD032328 | Pride | 2023-05-05

REPOSITORIES: Pride

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			Action	DRS
	200319_Fusion__LC2__NewObj_AH_mIMCD3_tryp_WT_126_127__TMEM260_KO1_128_129__KO2_130_131__ConA_2h_ETD_HCD_12ul_High_pH_1.raw	Raw
	200319_Fusion__LC2__NewObj_AH_mIMCD3_tryp_WT_126_127__TMEM260_KO1_128_129__KO2_130_131__ConA_2h_ETD_HCD_12ul_High_pH_2.raw	Raw
	200319_Fusion__LC2__NewObj_AH_mIMCD3_tryp_WT_126_127__TMEM260_KO1_128_129__KO2_130_131__ConA_2h_ETD_HCD_12ul_High_pH_3.raw	Raw
	200319_Fusion__LC2__NewObj_AH_mIMCD3_tryp_WT_126_127__TMEM260_KO1_128_129__KO2_130_131__ConA_2h_ETD_HCD_12ul_High_pH_3_HCD_raw_Semi_.msf	Msf
	200319_Fusion__LC2__NewObj_AH_mIMCD3_tryp_WT_126_127__TMEM260_KO1_128_129__KO2_130_131__ConA_2h_ETD_HCD_12ul_High_pH_4.raw	Raw

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