Dataset Information

Identification of ubquitination sites in SH3P2

ABSTRACT: Identification of ubiquitination sites in SH3P2 upon ubiquitin reaction with bacterial E3 ligase XopL

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Xanthomonas Campestris Pv. Vesicatoria Str. 85-10 Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Mirita Franz-Wachtel

LAB HEAD: Suayb Üstün

PROVIDER: PXD032375 | Pride | 2022-08-12

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20210222_CO_0997SuUe_R01.raw	Raw
	20210222_CO_0997SuUe_R02.raw	Raw
	20210222_CO_0997SuUe_R03.raw	Raw
	20210222_CO_0997SuUe_R04.raw	Raw
	20210222_CO_0997SuUe_R05.raw	Raw

Items per page:

1 - 5 of 7

Publications

A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component.

Leong Jia Xuan JX Raffeiner Margot M Spinti Daniela D Langin Gautier G Franz-Wachtel Mirita M Guzman Andrew R AR Kim Jung-Gun JG Pandey Pooja P Minina Alyona E AE Macek Boris B Hafrén Anders A Bozkurt Tolga O TO Mudgett Mary Beth MB Börnke Frederik F Hofius Daniel D Üstün Suayib S

The EMBO journal 20220527 13

Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unk ...[more]

PMID: 35620914

Similar Datasets

Project description:Xanthomonas is one important model microbe to study the molecular determinants of virulence and host range of pathogens since Xanthomonas is capable of infecting numerous monocotyledonous and dicotyledonous plants. Among the plant diseases caused by Xanthomonas, X. citri subsp. citri (Xcc) causes citrus canker, which has significant impact on citrus production. Xcc is classified into different strains primarily by host range including A and Aw. The A (Asiatic) strain (XccA) has a wide host range and is most virulent, whereas Aw (Wellington) strain has restricted host range including Key or Mexican lime and alemow. We hypothesized that not only gene content but also gene expression contributes to the difference in virulence and host range of closely related strains. To test our hypothesis, comparative genomic and transcriptome analyses were conducted to study the two closely related Xcc A and Aw strains. The genome of X. citri subsp. citri strain Aw12879 (Xcaw) was completely sequenced using 454 Pyrosequencing, Illumina sequencing and Optical mapping. The finished genome (5.3 Mb chromosome and two plasmids pXcaw19 and pXcaw58) of Xcaw was annotated, curated and compared with XccA genome. Protein blast revealed multiple genes including type III secretion system (TIIISS) effectors xopAF and xopAG are present in Xcaw but absent in XccA. Comparative genomic analysis showed various changes in genes encoding LPS and type IV secretion system. Furthermore, RNA-Seq was used to compare expression profile of Xcaw and XccA in nutrient rich (NB) medium and XVM2 medium which is known to mimic the intercellular space of plant cells using Illumina sequencing. Multiple avirulence/effector genes were over-expressed in Xcaw compared to XccA which might contribute to the limited host range of Xcaw compared to XccA. The overexpression of genes involved in cell wall degradation, attachment, ROS (reactive oxygen species) scavenging, nutrient transportation in XccA might contribute to its expanding of host range. Our data suggest that both gene content and gene expression contribute to difference in virulence and host range of bacterial pathogens. This study lays the foundation to further characterize the mechanisms for virulence and host range of strains of X. citri subsp. citri and other bacterial pathogens. mRNA expression profiles of Xcc strain A and Aw were generated in 2 media: NB and XVM2 by deep sequencing, in triplicate, using Illumina GAII.