Dataset Information

Polyphenols and IUGR Pregnancies: Effects of the Antioxidant Hydroxytyrosol on the Hippocampus Proteome in a Porcine Model

ABSTRACT: Supplementation of the mother's diet with antioxidants such as hydroxytyrosol (HTX) has been proposed to ameliorate adverse phenotypes of foetuses affected by intrauterine growth restriction (IUGR). Our previous studies showed, in a porcine model of IUGR, an effect of maternal HTX supplementation on the neurotransmitter profile of several brain areas and the morphology of the hippocampus in 100-days old foetuses. The present study analyzed the impact of maternal HTX supplementation on the hippocampus proteome at this foetal age by TMT10plex labelling. Eleven differentially abundant proteins were identified comparing both conditions, eight of them downregulated and three upregulated in the HTX-treated group. Downregulated proteins were mainly involved in protein synthesis and RNA metabolism, and may explain the differences in neuron differentiation in the HTX-treated group. Upregulated proteins were related to cell detoxification and could represent a potential mechanism to explain the neuroprotective effect of HTX.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Brain

SUBMITTER: Montserrat Carrascal

LAB HEAD: Anna Bassols

PROVIDER: PXD033684 | Pride | 2022-08-12

REPOSITORIES: Pride

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Polyphenols and IUGR Pregnancies: Effects of the Antioxidant Hydroxytyrosol on the Hippocampus Proteome in a Porcine Model.

Yeste Natalia N Pérez-Valle Jorge J Vázquez-Gómez Marta M García-Contreras Consolación C González-Bulnes Antonio A Bassols Anna A

Antioxidants (Basel, Switzerland) 20220609 6

Supplementation of a mother's diet with antioxidants such as hydroxytyrosol (HTX) has been proposed to ameliorate the adverse phenotypes of foetuses affected by intrauterine growth restriction (IUGR). Our previous studies showed, in a porcine model of IUGR, an effect of maternal HTX supplementation on the neurotransmitter profile of several brain areas and the morphology of the hippocampus in 100 days old foetuses. The present study analyzed the impact of maternal HTX supplementation on the hipp ...[more]

PMID: 35740029

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Project description:Intrauterine Growth Restriction (IUGR) is a pathological condition that hinders the correct growth of the foetus during pregnancy due to oxygen or nutrient deficiency. Consequently, the foetus adapts its metabolism and physiology to survive in such scarce environment. The major adaptation is the so called “brain sparing”; this effect gives priority to brain development to ensure the individual survival. Nevertheless, this does not warrant the normal development of the brain and the risk exists of neurological and cognitive deficits at short or long term. In turn, this adaptation leads to other systemic alterations that affect the energetic metabolism, thus inducing the emergence of a fairly characterized phenotype called “thrifty phenotype”. This phenotype is responsible for the metabolic alterations that last up until adulthood, which increase the incidence of some diseases like diabetes and metabolic syndrome. On the other hand, diets rich in fat are known to exert pernicious effects Using a pig model of IUGR, animals are classified as normal birth weight (NBW) or low birth weight (LBW). We have studied the effect of a long-term high fat diet (HFD) on the neurological alterations regarding the neurotransmitter profile in several brain areas, the morphology of the hippocampus and the proteome of this same brain area. Our hypothesis is that those animals that were affected by IUGR during their gestation, and therefore were born LBW, will present a different susceptibility to a HFD than NBW animals when they become adults. Our results indicate that HFD had a significant effect on the neurotransmitter profile of the hippocampus, amygdala, hypothalamus, striatum and prefrontal cortex. The most affected neurotransmitter was serotonin (5-HT), thus affecting the indolamine pathway. On the other hand, HFD does not provoke relevant changes in the morphology of the hippocampus. Finally, the proteomic analysis revealed that, in some instances, NBW and LBW animals respond to HFD in different ways. In particular, NBW animals present changes in the mitochondrial respiratory chain and oxidative phosphorylation, and in the extracellular matrix and its interaction with the cell. LBW animals present differences in RNA splicing, anterograde and retrograde transport and the mTOR pathway.

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