Proteomics

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Proteomic analysis of rabbit fresh and cryopreserved semen (spermatozoa)

ABSTRACT: Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been investigated previously. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit spermatozoa. Comparative analysis and identification of proteins were performed using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. We found that 108 proteins differed in abundance in spermatozoa between fresh and frozen semen. Most of these proteins was involved in pathways related to energy metabolism and protein quality control under stress conditions, reproductive processes and mechanisms of cell death/survival regulation, resulting in a significant decrease of motility and viability of post-thawing rabbit sperm and its potential fertilizing ability.

INSTRUMENT(S): autoflex

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Spermatozoon, Sperm

SUBMITTER: Mariola Słowińska  

LAB HEAD: Mariola Słowińska

PROVIDER: PXD034832 | Pride | 2022-10-14

REPOSITORIES: Pride

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spot_1002_rabbit.dat Other
spot_1002_rabbit.mgf Mgf
spot_1002_rabbit.zip Other
spot_1005_rabbit.dat Other
spot_1005_rabbit.mgf Mgf
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Proteomic analysis of rabbit fresh and cryopreserved semen provides an important insight into molecular mechanisms of cryoinjuries to spermatozoa.

Rusco Giusy G   Słowińska Mariola M   Di Iorio Michele M   Cerolini Silvia S   Maffione Angela B AB   Ciereszko Andrzej A   Iaffaldano Nicolaia N  

Theriogenology 20220805

Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been previously investigated. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit semen (spermatozoa and extracellular fluid), to examine the effects of freeze-thawing on proteins  ...[more]

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