Proteomics

Dataset Information

0

Proteomic analysis of rabbit fresh and cryopreserved semen (spermatozoa)

ABSTRACT: Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been investigated previously. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit spermatozoa. Comparative analysis and identification of proteins were performed using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. We found that 108 proteins differed in abundance in spermatozoa between fresh and frozen semen. Most of these proteins was involved in pathways related to energy metabolism and protein quality control under stress conditions, reproductive processes and mechanisms of cell death/survival regulation, resulting in a significant decrease of motility and viability of post-thawing rabbit sperm and its potential fertilizing ability.

INSTRUMENT(S): autoflex

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Spermatozoon, Sperm

SUBMITTER: Mariola Słowińska  

LAB HEAD: Mariola Słowińska

PROVIDER: PXD034832 | Pride | 2022-10-14

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
spot_1002_rabbit.dat Other
spot_1002_rabbit.mgf Mgf
spot_1002_rabbit.zip Other
spot_1005_rabbit.dat Other
spot_1005_rabbit.mgf Mgf
Items per page:
1 - 5 of 582
altmetric image

Publications

Proteomic analysis of rabbit fresh and cryopreserved semen provides an important insight into molecular mechanisms of cryoinjuries to spermatozoa.

Rusco Giusy G   Słowińska Mariola M   Di Iorio Michele M   Cerolini Silvia S   Maffione Angela B AB   Ciereszko Andrzej A   Iaffaldano Nicolaia N  

Theriogenology 20220805

Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been previously investigated. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit semen (spermatozoa and extracellular fluid), to examine the effects of freeze-thawing on proteins  ...[more]

Similar Datasets

Proteomics Proteomic analysis of rabbit fresh and cryopreserved semen (seminal plasma)
2022-10-13 | PXD034853 | Pride
Proteomics Proteomic analysis of fresh and liquid stored turkey spermatozoa
2024-01-26 | PXD043050 | Pride
Proteomics Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio) semen
2018-03-23 | PXD008187 | Pride
Proteomics Hormonal stimulation of carp is accompanied with changes of seminal plasma and spermatozoa proteome
2021-04-20 | PXD011049 | Pride
Proteomics Application of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Squamous Cell Carcinoma Subtype
2022-05-20 | PXD032962 | Pride
Proteomics Application of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Adenocarcinoma Subtype
2022-05-20 | PXD032736 | Pride
Proteomics Hypoxia induces Foxn1 and Mmp-9 expression in keratinocytes: proteomics analysis of mouse keratinocytes transiently activated by Foxn1
2018-04-09 | PXD007843 | Pride
Proteomics Identification of multiple transferrin species in spleen from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with the disease activity. Role of CD38. Set 2 of identified proteins (MALDI TOF/TOF UltrafleXtreme, Bruker).
2015-12-10 | PXD002643 | Pride
Proteomics Distinct Serum Proteome Profiles Associated with Collagen-induced Arthritis, and Complete Freund’s Adjuvant-induced Inflammation in CD38-/- mice: the Discriminative Power of Protein Species or Proteoforms. Spot 269_Spot 500
2015-07-17 | PXD002071 | Pride
Proteomics Identification of multiple transferrin species in spleen from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with the disease activity. Role of CD38. Citrullinated Proteins
2015-12-11 | PXD003183 | Pride