Proteomics

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Proteomic analysis of rabbit fresh and cryopreserved semen (seminal plasma)

ABSTRACT: Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been investigated previously. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit extracellular fluid of semen. Comparative analysis and identification of proteins were performed using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. We found that 28 proteins differed in abundance between fresh and frozen extracellular fluid.

INSTRUMENT(S): autoflex

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Seminal Plasma

SUBMITTER: Mariola Słowińska  

LAB HEAD: Mariola Słowińska

PROVIDER: PXD034853 | Pride | 2022-10-13

REPOSITORIES: Pride

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Source:
Action DRS
spot_1032_rabbit.dat Other
spot_1032_rabbit.mgf Mgf
spot_1032_rabbit.zip Other
spot_1050_rabbit.dat Other
spot_1050_rabbit.mgf Mgf
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Publications

Proteomic analysis of rabbit fresh and cryopreserved semen provides an important insight into molecular mechanisms of cryoinjuries to spermatozoa.

Rusco Giusy G   Słowińska Mariola M   Di Iorio Michele M   Cerolini Silvia S   Maffione Angela B AB   Ciereszko Andrzej A   Iaffaldano Nicolaia N  

Theriogenology 20220805

Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been previously investigated. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit semen (spermatozoa and extracellular fluid), to examine the effects of freeze-thawing on proteins  ...[more]

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