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Bacillus cereus biofilm formation with the aromatic amino acid L-tyrosine

ABSTRACT: The aim of this study was to identify and elaborate on the importance of the shikimate pathway, and specifically the aromatic amino acid L-tyrosine derived from it, for the formation of (air-liquid) biofilm of the food pathogen Bacillus cereus.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD036988 | Pride | 2023-10-03

REPOSITORIES: Pride

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			Action	DRS
	MQ_result_TA268-299.zip	Other
	TA268_WTglu_SupA.raw	Raw
	TA269_WTty_SupA.raw	Raw
	TA270_39glu_SupA.raw	Raw
	TA271_39ty_SupA.raw	Raw

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Publications

l-tyrosine modulates biofilm formation of Bacillus cereus ATCC 14579.

Huijboom Linda L Tempelaars Marcel M Fan Mingzhen M Zhu Yourong Y Boeren Sjef S van der Linden Erik E Abee Tjakko T

Research in microbiology 20230418 6

Bacillus cereus is a food-borne pathogen capable of producing biofilms. Following analysis of biofilm formation by B. cereus ATCC 14579 transposon mutants in defined medium (DM), a deletion mutant of bc2939 (Δbc2939) was constructed that showed decreased crystal violet biofilm staining and biofilm cell counts. In addition, Δbc2939 also produced smaller colony biofilms with lower cell counts and loss of wrinkly morphology. The bc2939 gene encodes for Prephenate dehydrogenase, which converts Preph ...[more]

PMID: 37080258

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