Dataset Information

Proteolytic processing of galectin-3 is crucial for host microbiome homeostasis

ABSTRACT: To identify relevant substrates for meprin α/β heterodimers, we applied mass spectrometry-based N-terminomics of mouse colon tissue as well as of human Caco-2 cells and identified galectin-3 as a novel intestinal substrate. The proteolytic processing of galectin-3 by meprin α/β heterodimers plays a key role in the maintenance of the host-microbiome homeostasis within the colon.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon

SUBMITTER: Andreas Tholey

LAB HEAD: Andreas Tholey

PROVIDER: PXD037000 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	A9_04022022_m1_0_CHY.pdResult	Other
	A9_04022022_m1_0_CHY.raw	Raw
	A9_04022022_m1_0_TRY.pdResult	Other
	A9_04022022_m1_0_TRY.raw	Raw
	A9_04022022_m2_0_CHY.pdResult	Other

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Proteolytic processing of galectin-3 by meprin metalloproteases is crucial for host-microbiome homeostasis.

Bülck Cynthia C Nyström Elisabeth E L EEL Koudelka Tomas T Mannbar-Frahm Michael M Andresen Gerrit G Radhouani Mariem M Tran Florian F Scharfenberg Franka F Schrell Friederike F Armbrust Fred F Dahlke Eileen E Zhao Bei B Vervaeke Alex A Theilig Franziska F Rosenstiel Philip P Starkl Philipp P Rosshart Stephan P SP Fickenscher Helmut H Tholey Andreas A Hansson Gunnar C GC Becker-Pauly Christoph C

Science advances 20230331 13

The metalloproteases meprin α and meprin β are highly expressed in the healthy gut but significantly decreased in inflammatory bowel disease, implicating a protective role in mucosal homeostasis. In the colon, meprin α and meprin β form covalently linked heterodimers tethering meprin α to the plasma membrane, therefore presenting dual proteolytic activity in a unique enzyme complex. To unravel its function, we applied N-terminomics and identified galectin-3 as the major intestinal substrate for ...[more]

PMID: 37000885

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