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Human - Cells and Extracellular vesicles of Hela and MNT-1 WT vs CD63 KO Proteomes 2

ABSTRACT: CD63 is a tetraspanin, which organize on cholesterol enriched microdomains, especially on late endosomes. Previous work of our team has shown that CD63 could regulate the sorting of cargoes into ILVs through an ESCRT-independent manner in melanocytes. However, CD63 has also been involved in other trafficking events in other cell type and so far, we have no clear idea of the general process that CD63 would control and would unify these disparate reports. In order to elucidate to general role of CD63 we compared two cell lines, Hela and MNT-1 that we Knock-out for CD63 by CRISPR in both cell lines and investigated the EV generation and the expression level of proteins involved in this process in cell lysates and the EV pellet obtained by SEC.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lymph Node, Hela Cell

SUBMITTER: Vanessa Masson

LAB HEAD: Damarys Loew

PROVIDER: PXD037147 | Pride | 2024-04-19

REPOSITORIES: Pride

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			Action	DRS
	Design_PRIDE_v1_2022_MC.xlsx	Xlsx
	X2523FD.msf	Msf
	X2523FD.raw	Raw
	X2524FD.msf	Msf
	X2524FD.raw	Raw

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Human - Cells and Extracellular vesicles of Hela and MNT-1 WT vs CD63 KO Proteomes 1

Project description:CD63 is a tetraspanin, which organize on cholesterol enriched microdomains, especially on late endosomes. Previous work of our team has shown that CD63 could regulate the sorting of cargoes into ILVs through an ESCRT-independent manner in melanocytes. However, CD63 has also been involved in other trafficking events in other cell type and so far, we have no clear idea of the general process that CD63 would control and would unify these disparate reports. In order to elucidate to general role of CD63 we compared two cell lines, Hela and MNT-1 that we Knock-out for CD63 by CRISPR in both cell lines and investigated the EV generation and the expression level of proteins involved in this process in cell lysates and the EV pellet obtained by SEC.

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