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CSE1L and NUP37 proximity labeling in MCF10A cells expressing chimeric ErbB1:ErbB2 receptors

ABSTRACT: This project uses an MCF10A clone (B2B1) expressing chimeric synthetic receptors for ErbB1 (EGFR) and ErbB2 (HER2) along with BirA*-fused inducible alleles of CSE1L or NUP37. Cells were cultured with reconstituted basement membrane, BirA* alleles induced with doxycycline, ErbB receptors dimerized with a synthetic small molecule, and biotinylation was performed for 24 hours. Lysates underwent a two-step biotin affinity purification before gel electrophoresis and mass spectrometry on selected gel fragments.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Breast Cell Line

DISEASE(S): Breast Cancer

SUBMITTER: Kevin Janes

LAB HEAD: Kevin Allyn Janes

PROVIDER: PXD038327 | Pride | 2023-03-22

REPOSITORIES: Pride

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			Action	DRS
	BAF_LW_2122_BirA_CSE1L-1.mzid.gz	Mzid
	BAF_LW_2122_BirA_CSE1L-1.mzid_BAF_LW_2122_BirA_CSE1L-1.MGF	Mzid
	checksum.txt	Txt
	q181030q06.mzid.gz	Mzid
	q181030q06.mzid_q181030q06.MGF	Mzid

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