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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of hyperthermophilic crenarchaeum Thermoproteus tenax to compare autotrophic growth on CO2/H2 versus heterotrophic growth on glucose using a focussed central carbohydrate metabolism DNA microarray

ABSTRACT: In order to unravel the role of regulation on transcript level in the central carbohydrate metabolism (CCM) of Thermoproteus tenax (glycolytic/gluconeogenic carbon switch), the focused DNA microarray was constructed by using ORFs supposed to be involved in the CCM pathways. Transcriptional profiling was performed comparing autotrophic growth on CO2/H2 versus heterotrophic growth on glucose.

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Thermoproteus tenax

SUBMITTER: Melanie Zaparty

PROVIDER: E-MEXP-1376 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.

Zaparty Melanie M Zaigler Alexander A Stamme Claudia C Soppa Jörg J Hensel Reinhard R Siebers Bettina B

Journal of bacteriology 20080104 6

In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed. ...[more]

PMID: 18178743

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