Dataset Information

The mass spectrometry-based proteomics analysis of macrophages

ABSTRACT: Defective autophagy is linked to proinflammatory diseases. However, the mechanisms by which autophagy limits inflammation remain elusive. Here, we found that the pan-FGFR inhibitor LY2874455 efficiently activated autophagy and suppressed innate inflammation in macrophages stimulated by lipopolysaccharide (LPS). The mass spectrometry-based proteomics analysis of RAW264.7 cells treated with LPS (20ng/ml) and LY2874455 was performed. The protein levels of macrophages were systematically analyzed and quantified. Then, the protein levels were compared. The fold change and significance were analyzed. Multiplex proteomic profiling identified the immunoproteasome, a specific isoform of the 20s constitutive proteasome, as substrates that are degraded by selective autophagy. SQSTM1/p62 was found to be a selective autophagy-related receptor that mediated this degradation. Autophagy deficiency or p62 knockdown blocked the effects of LY2874455, leading to the accumulation of immunoproteasomes and increases in inflammatory reactions.

INSTRUMENT(S): timsTOF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood

SUBMITTER: Kefeng Lu

LAB HEAD: Kefeng Lu

PROVIDER: PXD038747 | Pride | 2024-05-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	SA091LQ_ctl_Slot2-10_1_6500.d.zip	Other
	SA091LQ_lps_Slot2-11_1_6502.d.zip	Other
	SA091LQ_lps_b6_Slot2-12_1_6504.d.zip	Other
	checksum.txt	Txt
	txt.zip	Other

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Publications

Pharmacological induction of autophagy reduces inflammation in macrophages by degrading immunoproteasome subunits.

Zhou Jiao J Li Chunxia C Lu Meng M Jiang Gaoyue G Chen Shanze S Li Huihui H Lu Kefeng K

PLoS biology 20240306 3

Defective autophagy is linked to proinflammatory diseases. However, the mechanisms by which autophagy limits inflammation remain elusive. Here, we found that the pan-FGFR inhibitor LY2874455 efficiently activated autophagy and suppressed expression of proinflammatory factors in macrophages stimulated by lipopolysaccharide (LPS). Multiplex proteomic profiling identified the immunoproteasome, which is a specific isoform of the 20s constitutive proteasome, as a substrate that is degraded by selecti ...[more]

PMID: 38447109

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