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Proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea

ABSTRACT: Essential oils (EOs) have been demonstrated as efficacious against B. cinerea. However, the underpinning enzymatic and proteomic mechanism for these inhibitory effects is not fully understood. This study investigated the effects of lemon (Le) and lemongrass (Lg) EOs (individually and in combination) against B. cinerea based on enzymatic and proteomic analyses.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Botryotinia Fuckeliana (noble Rot Fungus) (botrytis Cinerea) Botrytis Cinerea

TISSUE(S): Mycelium

SUBMITTER: Ashwil Klein

LAB HEAD: Ashwil Klein

PROVIDER: PXD038894 | Pride | 2023-01-18

REPOSITORIES: pride

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			Action	DRS
	201912_1_191218081915.mzid.gz	Mzid
	201912_AK-11.mzid.gz	Mzid
	201912_AK-11.mzid_201912_AK-11.MGF	Mzid
	201912_AK-1_191218081915.mzid_201912_AK-1_191218081915.MGF	Mzid
	201912_AK-6.mzid.gz	Mzid

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Comparative proteomic analysis of the exoproteome of a wild type and cps1 mutant strains of the filamentous fungus Botrytis cinerea.

Project description:Botrytis cinerea is the causing agent of grey mould on hundreds of plants cultivated in fields, tunnels and greenhouses worldwide, including economically important crops. Considering the central role of the fungal cell wall in the interaction between fungi and plants, the role of a conserved putative polysaccharide synthase in the physiology, development and virulence of B. cinerea was explored. To this aim, the BcCps1 gene was deleted and the mutant strain was characterized. In order to reveal the impact of the mutation on the fungal secretion, the exo-proteome of the wild type and mutant strains were prepared for comparative analysis of their contents.

2022-10-15 | PXD033590 | Pride

vg120629 - Beyond plant defense: insights on the potential of salicylic and methylsalicylic acid to contain growth of the phytopathogen Botrytis cinerea

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Next Generation Sequencing Facilitates Quantitative Analysis of Cucumber and Botrytis cinerea Transcriptome Changes During Infection

Project description:Purpose: The goals of this study are using RNA-seq to obtain cucumber and Botrytis cinerea transcriptome changes during infection Methods: mRNA profiles of anti-infection samples and interaction sample were generate by deep sequencing,using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: BurrowsâWheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRTâPCR validation was performed using SYBR Green assays Results: Using an optimized data analysis workflow,In total, 248,908,688 raw reads were generated; after removing low-quality reads and those containing adapter and poly-N, 238,341,648 clean reads remained to map the reference genome. There were 3,512 cucumber (differential expression genes) DEGs and 1,735 B. cinerea DEGs. GO enrichment and KEGG enrichment analysis were performed on these DEGs to study the interaction between cucumber and B. cinerea. To verify the reliability and accuracy of our transcriptome data, 5 cucumber DEGs and 5 B. cinerea DEGs were chosen for RT-PCR verification. Conclusions:To the best of our knowledge, this is the first analysis of large-scale transcriptome changes of cucumber during the infection of Botrytis cinerea. These results will increase our understanding of the molecular mechanisms of the cucumber defense Botrytis cinerea and may be used to protect plants against disasters caused by necrotrophic fungal pathogens. mRNA profiles of infection and anti-infection cucumber were generated by deep sequencing, using Illumina Hiseq 2500 .

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Proteomic analysis of the extracellular vesicles of the filamentous fungus Botrytis cinerea.

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Unravelling the initial triggers of Botrytis cinerea infections. Botrytis cinerea surfactome

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Proteomic analysis of the proteins secreted by the infection cushions of Botrytis cinerea.

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Proteomic analysis of of the exoproteome of a clathrin mutant

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