The Triticale Mature Pollen and Stigma Proteomes – Assembling the Proteins for a Productive Encounter

ABSTRACT: Triticeae crops are major contributors to global food production and ensuring their capacity to reproduce and generate seeds is critical. However, despite their importance our knowledge of the proteins underlying Triticeae reproduction is severely lacking and this is not only true of pollen and stigma development, but also of their pivotal interaction. When the pollen grain and stigma are brought together they have each accumulated the proteins required for their intended meeting and accordingly studying their mature proteomes is bound to reveal proteins involved in their diverse and complex interactions. Using triticale as a Triticeae representative, gel-free shotgun proteomics was used to identify 11533 and 2977 mature stigma and pollen proteins respectively. These datasets, by far the largest to date, provide unprecedented insights into the proteins participating in Triticeae pollen and stigma development and interactions. The study of the Triticeae stigma has been particularly neglected and to begin filling this knowledge gap, a developmental iTRAQ analysis was performed revealing 647 proteins displaying differential abundance as the stigma matures in preparation for pollination. An in-depth comparison to an equivalent Brassicaceae analysis divulged both conservation and diversification in the makeup and function of proteins involved in the pollen and stigma encounter.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): X Triticosecale

TISSUE(S): Stigma, Pollen

SUBMITTER: Renee Robinson  

LAB HEAD: Dr. Laurian Robert

PROVIDER: PXD038905 | Pride | 2023-03-08

REPOSITORIES: Pride