Dataset Information

Cleavage of rTLP and rCHI by Peptidases from Drosophila suzukii Larval (Peptdomics Analysis)

ABSTRACT: We produced recombinant TLP and CHI as model thermolabile wine haze proteins and applied a peptidomics strategy to investigate whether D. suzukii larval peptidases were able to digest them under acidic conditions (pH 3.5) typically found in winemaking practices.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Cell Culture

SUBMITTER: Wendell Albuquerque

LAB HEAD: Wendell Albuquerque

PROVIDER: PXD039025 | Pride | 2023-05-10

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	AEX_proteomics_peptidases.raw	Raw
	CHI_DS_Larvae_ph3.5.raw	Raw
	SEC_proteomics_peptidases.raw	Raw
	TLP_DS_Larvae_ph3.5.raw	Raw
	supplementary_data_1_suzukii.xlsx	Xlsx

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Publications

Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae.

Albuquerque Wendell W Ghezellou Parviz P Lee Kwang-Zin KZ Schneider Quintus Q Gross Phillip P Kessel Tobias T Omokungbe Bodunrin B Spengler Bernhard B Vilcinskas Andreas A Zorn Holger H Gand Martin M

Biomolecules 20230228 3

Thermolabile grape berry proteins such as thaumatin-like proteins (TLPs) and chitinases (CHIs) promote haze formation in bottled wines if not properly fined. As a natural grapevine pest, the spotted-wing fly <i>Drosophila suzukii</i> is a promising source of peptidases that break down grape berry proteins because the larvae develop and feed inside mature berries. Therefore, we produced recombinant TLP and CHI as model thermolabile wine haze proteins and applied a peptidomics strategy to investig ...[more]

PMID: 36979386

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