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Proteomes of vaginal fluids during the estrous cycle in wild house mice.

ABSTRACT: We provide proteomes of vaginal fluid in three phases of the estrous cycle – proestrus, estrus and metestrus in six adult females of wild mice Mus musculus musculus. The total of 1167 particular proteins were identified by label-free nano- scale liquid chromatography tandem mass spectrometry (nLC-MS 2 ).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Vaginal Fluid

SUBMITTER: Pavel Stopka

LAB HEAD: Pavel Stopka

PROVIDER: PXD039239 | Pride | 2024-05-24

REPOSITORIES: Pride

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Publications

Microbial, proteomic, and metabolomic profiling of the estrous cycle in wild house mice.

Matějková Tereza T Dodoková Alica A Kreisinger Jakub J Stopka Pavel P Stopková Romana R

Microbiology spectrum 20240103 2

Symbiotic microbial communities affect the host immune system and produce molecules contributing to the odor of an individual. In many mammalian species, saliva and vaginal fluids are important sources of chemical signals that originate from bacterial metabolism and may act as honest signals of health and reproductive status. In this study, we aimed to define oral and vaginal microbiomes and their dynamics throughout the estrous cycle in wild house mice. In addition, we analyzed a subset of vagi ...[more]

PMID: 38171017

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Mouse utero-tubular junction (UTJ): 1: females in estrous no mating; 2: females in estrous mated with vasectomized male; 3: females in estrous mated with normal male: 1 vs. 2; 1 vs. 3; 2 vs. 3.

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Sperm morphology and performance in relation to postmating prezygotic isolation in two recently diverged passerine species

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Messenger RNA profiling of bovine endometrium during the estrous cycle using a custom-made cDNA array

Project description:The endometrium provides optimal conditions for the transport of sperm to the oviduct, to the site of fertilization, and later on for the reception of the embryo. To study these changes on the level of gene expression, a messenger RNA expression profiling of endometrium tissue samples collected from 19 cyclic heifers at five stages of the estrous cycle (days 0, 3.5, 12, 18, 20) was performed. RNA was extracted from these tissue samples and analyzed with a custom-made bovine oviduct and endometrium (BOE) cDNA array. The cDNAs present on the array were derived from several previously conducted differential gene expression studies of bovine endometrium between different stages of the estrous cycle, during early pregnancy, and from studies of bovine oviduct epithelial cells. In all of these studies cDNAs of differentially expressed genes were identified using a combination of subtracted cDNA libraries and cDNA array hybridization. 1,440 cDNA fragments are located on the array. Twenty radioactively labeled cDNA samples (n=4 for each cycle stage) were hybridized with BOE arrays. Raw data were normalized using the BioConductor package vsn. Keywords: time course of gene expression in bovine endometrium during estrous cycle Nineteen German Fleckvieh (Simmental) heifers were slaughtered at four different days of the estrous cycle, four animals at day 0, four at day 3.5, three at day 12 and eight animals at day 18. Four of the day 18 animals showed high and the other four low serum progesterone (P4) levels, respectively. This resulted in five groups of endometrial tissue samples corresponding to five stages of the estrous cycle.

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Transcriptional response across estrous in the mouse dorsal hippocampus is dysregulated by Cnih3 mutation

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