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An isobaric labeling approach to enhance detection and quan-tification of tissue-derived plasma proteins as potential early disease biomarkers

ABSTRACT: Accurate detection and quantification of low abundance proteins by standard mass spectrometry approaches remains challenging. In addition, it is difficult to link low abundance plasma proteins back to their specific tissues or organs of origin with confidence. To address these challenges, we developed a mass spectrometry approach based on the use of tandem mass tags (TMT) and a tis-sue reference sample.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Papio Anubis Anubis Papio Hamadryas Adenovirus 1

TISSUE(S): Blood Plasma, Kidney

SUBMITTER: Sumaiya Nazli

LAB HEAD: Michael Olivier

PROVIDER: PXD039430 | Pride | 2023-03-11

REPOSITORIES: pride

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			Action	DRS
	10x1_MS2_TMT1.raw	Raw
	10x1_MS2_TMT2.raw	Raw
	10x1_MS2_TMT3.raw	Raw
	10x1_MS2_TMT4.raw	Raw
	10x1n2f1.raw	Raw

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Publications

An Isobaric Labeling Approach to Enhance Detection and Quantification of Tissue-Derived Plasma Proteins as Potential Early Disease Biomarkers.

Nazli Sumaiya S Zimmerman Kip D KD Riojas Angelica M AM Cox Laura A LA Olivier Michael M

Biomolecules 20230122 2

The proteomic analysis of plasma holds great promise to advance precision medicine and identify biomarkers of disease. However, it is likely that many potential biomarkers circulating in plasma originate from other tissues and are only present in low abundances in the plasma. Accurate detection and quantification of low abundance proteins by standard mass spectrometry approaches remain challenging. In addition, it is difficult to link low abundance plasma proteins back to their specific tissues ...[more]

PMID: 36830584

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A transient protein folding response targets aggregation in the early phase of TDP-43-mediated disease

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