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Proteomic profiling of eIF3a conditional knockout mice

ABSTRACT: In this study, a Cre-loxP system was used to generate eIF3a conditional knockout mice. Tandem mass tag (TMT) labeling with LC-MS/MS analysis was used to identify differentially expressed proteins (DEPs) in fat, lungs, skin, and spleen tissue of the eIF3a knockout mice and controls. Bioinformatics analysis was then used to explore the functions and molecular signaling pathways of these protein landscapes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Lung, Skin

SUBMITTER: Wei Zhuo

LAB HEAD: Zhaoqian Liu

PROVIDER: PXD041416 | Pride | 2023-07-20

REPOSITORIES: Pride

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Proteomic profiling of eIF3a conditional knockout mice.

Zhuo Wei W Chen Juan J Jiang Shilong S Zheng Juyan J Huang Hanxue H Xie Pan P Li Wei W Lei Mengrong M Yin Jiye J Gao Ying Y Liu Zhaoqian Z

Frontiers in molecular biosciences 20230419

Eukaryotic translation initiation factor 3 subunit A (eIF3a) is the largest subunit of the eukaryotic translation initiation factor 3 (eIF3). eIF3a plays an integral role in protein biosynthesis, hence impacting the onset, development, and treatment of tumors. The proteins regulated by eIF3a are still being explored <i>in vivo</i>. In this study, a Cre-loxP system was used to generate eIF3a conditional knockout mice. Tandem mass tag (TMT) labeling with LC-MS/MS analysis was used to identify diff ...[more]

PMID: 37152897

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