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A genetic deficiency screen in vivo reveals rescue mechanisms of mitochondrial dysfunction

ABSTRACT: The mitochondrial network forms a central hub in cellular metabolism, adapting to a vast array of environmental and cellular signals to maintain cellular homeostasis. Derangements to this network can lead to mitochondrial diseases but are also increasingly recognised to be a contributing factor in common disorders including heart failure, neurodegeneration, cancer, or the natural ageing process. Despite its central importance, little is known how the cell responds to a mitochondrial dysfunction, contributing to our lack of affective treatment strategies. Here we performed an unbiased genetic deficiency screen in the fruit fly to identify mechanisms that can rescue a lethal mitochondrial dysfunction. After screening ~40% of the fly genome, we identified 10 genes from four distinct cellular pathways that rescued the mitochondrial dysfunction upon heterozygous deletion, resulting in viable adult flies. The data presented here includes proteomic analysis of larvae of control, mutant, and rescued lines.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Brain, Whole Body, Fat Body

SUBMITTER: Mario Oroshi

LAB HEAD: Dr. Matthias Mann

PROVIDER: PXD041418 | Pride | 2024-12-01

REPOSITORIES: Pride

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			Action	DRS
	20220219_EXPL4_FAS_SA_S669_Dm_Br_DILP1_F5_rep1_Evo65_88min_40w.raw	Raw
	20220219_EXPL4_FAS_SA_S669_Dm_Br_DILP1_G5_rep2_Evo77_88min_40w.raw	Raw
	20220219_EXPL4_FAS_SA_S669_Dm_Br_DILP1_H5_rep3_Evo89_88min_40w.raw	Raw
	20220219_EXPL4_FAS_SA_S669_Dm_Br_Melt_A4_rep1_Evo4_88min_40w.raw	Raw
	20220219_EXPL4_FAS_SA_S669_Dm_Br_Melt_B4_rep2_Evo16_88min_40w.raw	Raw

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A genetic deficiency screen in vivo reveals rescue mechanisms of mitochondrial dysfunction

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