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Proteome data of a BP1092 mutant of Bordetella pertussis

ABSTRACT: Proteome data of a BP1092 mutant of Bordetella pertussis under virulence modulating and non-modulating conditions

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bordetella Pertussis (strain Tohama I / Atcc Baa-589 / Nctc 13251)

TISSUE(S): Cell Culture

SUBMITTER: Kristin Surmann

LAB HEAD: Uwe Völker

PROVIDER: PXD041940 | Pride | 2024-05-24

REPOSITORIES: Pride

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			Action	DRS
	20220502_HF_KRIS_BIOPIC_10_delta1092_SO4_BR2.raw	Raw
	20220502_HF_KRIS_BIOPIC_11_compl1092_BR2.raw	Raw
	20220502_HF_KRIS_BIOPIC_12_compl1092_SO4_BR2.raw	Raw
	20220502_HF_KRIS_BIOPIC_13_wt_BR3.raw	Raw
	20220502_HF_KRIS_BIOPIC_14_wt_SO4_BR3.raw	Raw

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Publications

Role of the Putative Histidine Kinase BP1092 in Bordetella pertussis Virulence Regulation and Intracellular Survival.

Debandi Martina M Carrica Mariela M Hentschker Christian C Baroli Carlos C Völker Uwe U Rodriguez Maria Eugenia ME Surmann Kristin K Lamberti Yanina Y

Journal of proteome research 20240422 5

Bordetella pertussis persists inside host cells, and virulence factors are crucial for intracellular adaptation. The regulation of B. pertussis virulence factor transcription primarily occurs through the modulation of the two-component system (TCS) known as BvgAS. However, additional regulatory systems have emerged as potential contributors to virulence regulation. Here, we investigate the impact of BP1092, a putative TCS histidine kinase that shows increased levels after bacterial ...[more]

PMID: 38644792

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Proteomics of Bordetella pertussis whole-cell and acellular vaccines

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Shotgun proteomic analysis of Bordetella pertussis response to iron availability

Project description:B. pertussis Tohama I was cultivated in iron-depleted or iron-repleted medium in order to monitor proteins which are influence by iron supply. More than 200 proteins displayed increased or decreased levels. Proteins involved in iron acquisition, biofilm production and oxidative stress response were increased during iron starvation. Limited iron supply caused on the other hand decreased levels of virulence factors regulated by the BvgAS system.

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BtcB (BP2265) is a T3SS chaperone of the CesT family in Bordetella pertussis

Project description:Bordetella pertussis is a Gram-negative, strictly human respiratory pathogen and the causative agent of whooping cough (pertussis). Similar to other Gram-negative pathogens, B. pertussis produces a functional type III secretion system, but its role in pathogenesis of B. pertussis is enigmatic and has not yet been elucidated. Here, we applied omics RNA-seq as well as LC-MS/MS techniques and co-immunoprecipitation method to identify and characterize the novel CesT family T3SS chaperone BP2265. Our results show that the chaperone BP2265 specifically interacts with the secreted T3SS anti-sigma factor BtrA. Moreover, in the absence of the chaperone, secretion but not production of BtrA and several early, intermediate, and late T3SS substrates is severely impaired. It appears that the role of BtrA in regulating T3SS is more complex and extends beyond its activity as an antagonist of the sigma factor BtrS. We propose to rename BP2265 as BtcB for the Bordetella type III chaperone of BtrA.

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Hfq is involved in global protein expression and stress response in Bordetella pertussis

Project description:In this study, we used gel-free nanoLC-MS/MS-based proteomics to compare the protein profile of B. pertussis wild type and an isogenic hfq defective mutant strain under control an iron limited conditions. We found that Hfq affects the abundance of 302 proteins, which represents 8% of the total B. pertussis coding sequence. The absence of Hfq induced changes in the abundance of proteins involved in metabolic pathways, stress response and virulence. Hfq was also found involved in B. pertussis adaptation to iron starvation, one of the main stresses this pathogen faces inside the host. Altogether, these results indicate that B. pertussis Hfq is involved in bacterial physiological processes as well as bacterial pathogenesis.

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Acetylome analysis of Bordetella pertussis reveals possible targets of novel BP0960 gene-encoded lysine deacetylase

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Integrating proteomic data with metabolic modelling provides insight into key pathways in Bordetella pertussis biofilms

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2023-08-07 | PXD033664 | Pride

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