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Shotgun proteomic analysis of Bordetella pertussis response to iron availability

ABSTRACT: B. pertussis Tohama I was cultivated in iron-depleted or iron-repleted medium in order to monitor proteins which are influence by iron supply. More than 200 proteins displayed increased or decreased levels. Proteins involved in iron acquisition, biofilm production and oxidative stress response were increased during iron starvation. Limited iron supply caused on the other hand decreased levels of virulence factors regulated by the BvgAS system.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bordetella Pertussis (strain Tohama I / Atcc Baa-589 / Nctc 13251)

SUBMITTER: Kristin Surmann

LAB HEAD: Frank Schmidt

PROVIDER: PXD001280 | Pride | 2015-03-16

REPOSITORIES: Pride

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			Action	DRS
	120304_O4_YL__Bp_Control_A.RAW	Raw
	120304_O4_YL__Bp_Control_A.xml	Xml
	120304_O4_YL__Bp_Control_C.RAW	Raw
	120304_O4_YL__Bp_Control_C.xml	Xml
	120305_O4_YL__Bp_Fe_limitation_2.RAW	Raw

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Publications

Shotgun proteome analysis of Bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response.

Alvarez Hayes Jimena J Lamberti Yanina Y Surmann Kristin K Schmidt Frank F Völker Uwe U Rodriguez Maria Eugenia ME

Proteomics 20150605 13

One of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. This study reports a gel-free nanoLC-MS/MS-based comparative proteome analysis of Bordetella pertussis grown under iron-excess and iron-depleted conditions. Out of the 926 proteins covered 98 displayed a shift in their abundance in response to low iron availability. Forty-seven of them were found to be increased in l ...[more]

PMID: 25755163

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