Proteomics

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Hfq is involved in global protein expression and stress response in Bordetella pertussis

ABSTRACT: In this study, we used gel-free nanoLC-MS/MS-based proteomics to compare the protein profile of B. pertussis wild type and an isogenic hfq defective mutant strain under control an iron limited conditions. We found that Hfq affects the abundance of 302 proteins, which represents 8% of the total B. pertussis coding sequence. The absence of Hfq induced changes in the abundance of proteins involved in metabolic pathways, stress response and virulence. Hfq was also found involved in B. pertussis adaptation to iron starvation, one of the main stresses this pathogen faces inside the host. Altogether, these results indicate that B. pertussis Hfq is involved in bacterial physiological processes as well as bacterial pathogenesis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bordetella Pertussis (strain Tohama I / Atcc Baa-589 / Nctc 13251)

SUBMITTER: Kristin Surmann  

LAB HEAD: Prof. Uwe Völker

PROVIDER: PXD011753 | Pride | 2020-02-18

REPOSITORIES: pride

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160621_OV1_MD_BP_C1_WT_control_BR1.raw Raw
160621_OV1_MD_BP_C2_WT_control_BR2.raw Raw
160621_OV1_MD_BP_C3_WT_control_BR3.raw Raw
160621_OV1_MD_BP_C4_WT_control_BR4.raw Raw
160621_OV1_MD_BP_D1_hfq_mutant_control_BR1.raw Raw
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Publications

B. pertussis is the etiological agent of whooping cough, a highly contagious respiratory disease which remains uncontrolled worldwide. Understanding how this pathogen responds to the environmental changes and adapts to different niches found inside the host might contribute to gain insight into bacterial pathogenesis. Comparative analyses of previous transcriptomic and proteomic data suggested that post-transcriptional regulatory mechanisms modulate B. pertussis virulence in response to iron ava  ...[more]

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