Proteomics

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Autophagy protein 5 controls flow-dependent endothelial functions


ABSTRACT: In this project two sets of proteomic experiments were performed: (1) Autophagosome content profiling: Autophagosomes were isolated from cultured primary lung endothelial cells of LC3-GFP and WT mice using anti-GFP uMACS (n=3 biological replicates). (2) Expression proteomics: Protein lysates from endothelial cells of n=3 young and old WT mice, young and old vecCRE-ATG5flox mice (ATG5 KO mice).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung, Lung Endothelial Cell

SUBMITTER: Joern Dengjel  

LAB HEAD: Joern Dengjel

PROVIDER: PXD042431 | Pride | 2023-08-07

REPOSITORIES: Pride

Dataset's files

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Action DRS
20200625_LD_Collab73_Autoph1_1.raw Raw
20200625_LD_Collab73_Autoph1_2.raw Raw
20200625_LD_Collab73_Autoph1_3.raw Raw
20200625_LD_Collab73_Autoph1_4.raw Raw
20200625_LD_Collab73_Ctrl1_1.raw Raw
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Publications


Dysregulated autophagy is associated with cardiovascular and metabolic diseases, where impaired flow-mediated endothelial cell responses promote cardiovascular risk. The mechanism by which the autophagy machinery regulates endothelial functions is complex. We applied multi-omics approaches and in vitro and in vivo functional assays to decipher the diverse roles of autophagy in endothelial cells. We demonstrate that autophagy regulates VEGF-dependent VEGFR signaling and VEGFR-mediated and flow-me  ...[more]

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