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Immunopeptidome of a human beta cell line infected by Coxsackievirus B strains

ABSTRACT: Coxsackievirus B (CVB) infection of pancreatic β cells is associated with β-cell autoimmunity. We investigated how CVB impacts human β cells and anti-CVB T-cell responses. β cells were efficiently infected by CVB in vitro, downregulated HLA Class I and presented few, selected HLA-bound viral peptides. Circulating CD8+ T cells from CVB-seropositive individuals recognized only a fraction of these peptides, and only another sub-fraction was targeted by effector/memory T cells that expressed the exhaustion marker PD-1. T cells recognizing a CVB epitope cross-reacted with the GAD β-cell antigen. Infected β cells, which formed filopodia to propagate infection, were more efficiently killed by CVB than by anti-CVB T cells. Thus, our in-vitro and ex-vivo data suggest limited T-cell responses to CVB, supporting the rationale for CVB vaccination trials for type 1 diabetes prevention. CVB-reactive CD8+ T cells provide biomarkers to follow response to infection and vaccination.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endocrine Pancreas, Pancreatic Endocrine Cell

SUBMITTER: Roberto Mallone

LAB HEAD: Roberto Mallone

PROVIDER: PXD042711 | Pride | 2023-08-19

REPOSITORIES: Pride

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			Action	DRS
	CVB1_protein-peptides.csv	Csv
	CVB3_1_protein-peptides.csv	Csv
	CVB3_2_protein-peptides.csv	Csv
	HF171017_1269_i_ac.raw	Raw
	HF180208_0139_i_asc.raw	Raw

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