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Eimeria necatrix Wall-Forming Bodies and Oocyst Wall Proteomic Analysis by Tandem Mass Tag (TMT) Labeling and Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) Techniques

ABSTRACT: WFBs and the oocyst wall of Eimeria necatrix were subjected to comparative proteomic analysis using tandem mass tag (TMT) in conjunction with liquid chromatography tandem-mass spectrometry (LC-MS/MS) techniques

INSTRUMENT(S): Q Exactive

ORGANISM(S): Eimeria Necatrix

SUBMITTER: Wang Lele

LAB HEAD: Lele Wang

PROVIDER: PXD042839 | Pride | 2024-01-26

REPOSITORIES: Pride

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Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix.

Wang Lele L Liu Dandan D Zhu Yu Y Wang Feiyan F Cai Weimin W Feng Qianqian Q Su Shijie S Hou Zhaofeng Z Xu Jinjun J Hu Junjie J Tao Jianping J

Parasites & vectors 20231218 1

<h4>Background</h4>The durable oocyst wall formed from the contents of wall-forming bodies (WFBs) protects Eimeria parasites from harsh conditions and enhances parasite transmission. Comprehending the contents of WFBs and proteins involved in oocyst wall formation is pivotal to understanding the mechanism of the oocyst wall formation and the search for novel targets to disrupt parasite transmission.<h4>Methods</h4>Total proteins extracted from WFBs and the oocyst wall of Eimeria necatrix were su ...[more]

PMID: 38111000

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