Dataset Information

Pericyte-specific secretome profiling in hypoxia using TurboID in an in vitro spheroid-model

ABSTRACT: This project utilizes TurboID to tag secreted proteins with biotin for subsequent cell specific analysis of the secretome from co-culture conditions

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Brain Pericyte

DISEASE(S): Disease Free

SUBMITTER: Charlotte Welinder

LAB HEAD: Andreas Enström

PROVIDER: PXD044689 | Pride | 2024-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AE_20221002_10_LW_X2211_021.mzML	Mzml
	AE_20221002_10_LW_X2211_021.raw	Raw
	AE_20221002_11_LW_X2211_023.mzML	Mzml
	AE_20221002_11_LW_X2211_023.raw	Raw
	AE_20221002_12_LW_X2211_025.mzML	Mzml

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Publications

Pericyte-Specific Secretome Profiling in Hypoxia Using TurboID in a Multicellular in Vitro Spheroid Model.

Enström Andreas A Carlsson Robert R Buizza Carolina C Lewi Marvel M Paul Gesine G

Molecular & cellular proteomics : MCP 20240504 6

Cellular communication within the brain is imperative for maintaining homeostasis and mounting effective responses to pathological triggers like hypoxia. However, a comprehensive understanding of the precise composition and dynamic release of secreted molecules has remained elusive, confined primarily to investigations using isolated monocultures. To overcome these limitations, we utilized the potential of TurboID, a non-toxic biotin ligation enzyme, to capture and enrich secreted proteins speci ...[more]

PMID: 38705386

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