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Proteotyping of sets of six microbial isolates by tandem mass spectrometry.

ABSTRACT: Proteotyping of mixtures of isolates was carried out by shotgun proteomics and phylopeptidomic interpretation to identify the microorganisms at the species taxonomical rank.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sulfitobacter Indolifex Sagittula Stellata Marivirga Tractuosa Ruegeria Pomeroyi Saccharomyces Cerevisiae (baker's Yeast) Klebsiella Aerogenes

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean ARMENGAUD

PROVIDER: PXD044755 | Pride | 2024-05-02

REPOSITORIES: Pride

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			Action	DRS
	CQDB_2023-08-21.fasta	Fasta
	F230539.dat	Other
	F230539_p0.05.mzid.gz	Mzid
	F230540.dat	Other
	F230540_p0.05.mzid.gz	Mzid

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Publications

A Simplified Label-Free Method for Proteotyping Sets of Six Isolates in a Single Liquid Chromatography-High-Resolution Tandem Mass Spectrometry Analysis.

Chabas Madisson M Armengaud Jean J Alpha-Bazin Béatrice B

Journal of proteome research 20240207 3

Clinical diagnostics and microbiology require high-throughput identification of microorganisms. Sample multiplexing prior to detection is an attractive means to reduce analysis costs and time-to-result. Recent studies have demonstrated the discriminative power of tandem mass spectrometry-based proteotyping. This technology can rapidly identify the most likely taxonomical position of any microorganism, even uncharacterized organisms. Here, we present a simplified label-free multiplexing method to ...[more]

PMID: 38327087

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