Proteomics

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Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1 (DDA method) followed by identification of phosphorylation on S/T

ABSTRACT: ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana lines expressing the Pseudomonas syringae type III effector AvrRpm1. Enriched proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Lennart Wirthmueller  

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD045550 | Pride | 2023-09-28

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
KSI211222_1_1ul.raw Raw
KSI211222_2_5_1ul.raw Raw
KSI211222_5_1ul.raw Raw
KSI230223_A1_Af1521_4A_1ul_Triggered.raw Raw
KSI230223_A1_Af1521_4A_2ul_Triggered.raw Raw
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