Proteomics

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Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector HopU1

ABSTRACT: ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana lines expressing the Pseudomonas syringae type III effector HopU1. A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Lennart Wirthmueller  

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD056608 | Pride | 2024-10-22

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
KSI140824_3_Col-0_G42E_1ul.raw Raw
KSI140824_3_Col-0_G42E_6ul.raw Raw
KSI140824_4_Col-0_1521_1ul.raw Raw
KSI140824_4_Col-0_1521_3ul.raw Raw
KSI140824_5_HopU1_1521_1ul.raw Raw
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