Proteomics

Dataset Information

0

Pseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3)

ABSTRACT: Nicotiana benthamiana tissue expressing either PLDGAMMA3-3xHA-StrepII or co-expressing AvrRpm1-3xFlag and PLDGAMMA3-3xHA-StrepII were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Nicotiana Benthamiana Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Lennart Wirthmueller  

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD056607 | Pride | 2024-10-22

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
KSI290124_13A-PLDG3_1521.raw Raw
KSI290124_13A-PLDG3_1521_4ul.raw Raw
KSI290124_14A-PLDG3_G42E.raw Raw
KSI290124_14A-PLDG3_G42E_6ul.raw Raw
KSI290124_15A-A1_PLDG3_1521.raw Raw
Items per page:
1 - 5 of 11

Similar Datasets

Proteomics Pseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI)
2023-09-28 | PXD045549 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector HopU1
2024-10-22 | PXD056608 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1 (2.5 ug Af1521 Macro, technical replicate 1)
2023-09-28 | PXD045544 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1 (5 ug Af1521 Macro, technical replicate 2)
2023-09-28 | PXD045545 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1
2023-09-28 | PXD045511 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1, DDA method (2.5 ug Af1521 Macro, technical replicate 1)
2023-09-28 | PXD045558 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1 (DDA method) followed by identification of phosphorylation on S/T
2023-09-28 | PXD045550 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1, DDA method
2023-09-28 | PXD045638 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector AvrRpm1, DDA method (5 ug Af1521 Macro, technical replicate 2)
2023-09-28 | PXD045548 | Pride
Proteomics Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2
2023-09-28 | PXD045780 | Pride