Proteomics

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Pseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3)

ABSTRACT: Nicotiana benthamiana tissue expressing either PLDGAMMA3-3xHA-StrepII or co-expressing AvrRpm1-3xFlag and PLDGAMMA3-3xHA-StrepII were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Nicotiana Benthamiana Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Lennart Wirthmueller  

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD056607 | Pride | 2024-10-22

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
KSI290124_13A-PLDG3_1521.raw Raw
KSI290124_13A-PLDG3_1521_4ul.raw Raw
KSI290124_14A-PLDG3_G42E.raw Raw
KSI290124_14A-PLDG3_G42E_6ul.raw Raw
KSI290124_15A-A1_PLDG3_1521.raw Raw
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