Proteomics

Dataset Information

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TNFα conditional knockout promotes myoblasts proliferation, differentiation and muscle regeneration

ABSTRACT: TNFα is a cytokine with multiple biological effects. It activates cascading signal pathways by binding to receptors on the cell membrane, regulating processes such as cell growth, differentiation, apoptosis, and inflammatory responses. The formation of skeletal muscle primarily relies on processes such as myoblasts proliferation, migration, differentiation, cytoskeletal rearrangement, and myotube fusion, which constitute a complex and coordinated process regulated by multiple genes. While research on TNFα has primarily focused on immunology, some studies suggest that TNFα also plays a physiological role in myogenesis, muscle repair, diseases, and atrophy.We isolated the nuclei (N) and cytoplasm (C) of flox and TNFα-CKO primary myoblasts in the early stages of differentiation and attempted to identify new targets in the TNFα signaling pathway.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle

SUBMITTER: Xin Hao  

LAB HEAD: Xin Hao

PROVIDER: PXD046525 | Pride | 2024-08-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
XA01734LQ_CKO1_C.raw Raw
XA01734LQ_CKO1_N.raw Raw
XA01734LQ_CKO2_C.raw Raw
XA01734LQ_CKO2_N.raw Raw
XA01734LQ_CKO3_C.raw Raw
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Publications

Tumor necrosis factor α deficiency promotes myogenesis and muscle regeneration.

Fu Yu Y   Nie Jing-Ru JR   Shang Peng P   Zhang Bo B   Yan Da-Wei DW   Hao Xin X   Zhang Hao H  

Zoological research 20240701 4

Tumor necrosis factor α (TNFα) exhibits diverse biological functions; however, its regulatory roles in myogenesis are not fully understood. In the present study, we explored the function of <i>TNFα</i> in myoblast proliferation, differentiation, migration, and myotube fusion in primary myoblasts and C2C12 cells. To this end, we constructed <i>TNFα</i> muscle-conditional knockout ( <i>TNFα</i>-CKO) mice and compared them with <i>flox</i> mice to assess the effects of <i>TNFα</i> knockout on skele  ...[more]

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