Dataset Information

Development of a Global Metabo-Lipid-Prote-omics Workflow to Compare Healthy Proximal and Distal Colonic Epithelium in Mice

ABSTRACT: A multimetabo-lipid-prote-omics workflow was developed to characterize the molecular interplay within proximal (PC) and distal (DC) colonic epithelium of healthy mice. This multiomics data set lays the foundation to better understand the two tissue types and can be used to study, for example, colon-related diseases like colorectal cancer or inflammatory bowel disease. First, the methyl tert-butyl ether extraction method was optimized, so that from a single tissue biopsy >350 reference-matched metabolites, >1850 reference-matched lipids, and >4500 proteins were detected by using targeted and untargeted metabolomics, untargeted lipidomics, and proteomics. Next, each omics-data set was analyzed individually and then merged with the additional omics disciplines to generate a deep understanding of the underlying complex regulatory network within the colon. Our data demonstrates, for example, differences in mucin formation, detected on substrate level as well as on enzyme level, and altered lipid metabolism by the detection of phospholipases hydrolyzing sphingomyelins to ceramides. In conclusion, the combination of the three mass spectrometry-based omics techniques can better entangle the functional and regional differences between PC and DC tissue compared to each single omics technique.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon

SUBMITTER: Christina Ludwig

LAB HEAD: Christina Ludwig

PROVIDER: PXD046811 | Pride | 2024-09-04

REPOSITORIES: Pride

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			Action	DRS
	BBM_695_P247_04_MAH_001.raw	Raw
	BBM_695_P247_04_MAH_002.raw	Raw
	BBM_695_P247_04_MAH_003.raw	Raw
	BBM_695_P247_04_MAH_004.raw	Raw
	BBM_695_P247_04_MAH_005.raw	Raw

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Development of a Global Metabo-Lipid-Prote-omics Workflow to Compare Healthy Proximal and Distal Colonic Epithelium in Mice.

Hemmati Maryam M Wudy Susanne I SI Hackbarth Franziska F Mittermeier-Kleßinger Verena K VK Coleman Olivia I OI Haller Dirk D Ludwig Christina C Dawid Corinna C Kleigrewe Karin K

Journal of proteome research 20240725 8

A multimetabo-lipid-prote-omics workflow was developed to characterize the molecular interplay within proximal (PC) and distal (DC) colonic epithelium of healthy mice. This multiomics data set lays the foundation to better understand the two tissue types and can be used to study, for example, colon-related diseases like colorectal cancer or inflammatory bowel disease. First, the methyl <i>tert</i>-butyl ether extraction method was optimized, so that from a single tissue biopsy >350 reference-mat ...[more]

PMID: 39052308

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