Dataset Information

Impact of fibrillar collagen scaffold on corneal stromal fibroblasts protein expression

ABSTRACT: The human cornea relies on its extracellular matrix (ECM) properties, with corneal stromal fibroblasts (CSFs) crucial for ECM remodeling. We studied the effects of nanofibrillar materials on CSFs using a decellularized human amniotic membrane (dHAM) and fibrillar collagen scaffold (FCS). FCS enhances focal adhesions (FAs), activating mechanotransduction signals through FAK autophosphorylation and YAP1 nuclear translocation. Nanofibrillar topography induces gene expression changes related to tissue remodeling and collagen production. Here, we performed shotgun proteomics analysis (DDA-PASEF in TimsToF Pro) to analyze the impact of FCS topography on CSFs protein expression.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratocyte

SUBMITTER: Arseniy Lobov

LAB HEAD: Natalia Mikhailova

PROVIDER: PXD048096 | Pride | 2024-09-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	D2H_Slot1-20_1_212.d.zip	Other
	D2H_Slot1-20_1_212.mgf	Mgf
	D2H_Slot1-20_1_224.d.zip	Other
	D2H_Slot1-20_1_224.mgf	Mgf
	D2H_Slot1-20_1_232.d.zip	Other

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Publications

Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates.

Zhurenkov Kirill E KE Lobov Arseniy A AA Bildyug Natalya B NB Alexander-Sinclair Elga I EI Darvish Diana M DM Lomert Ekaterina V EV Kriger Daria V DV Zainullina Bozhana R BR Chabina Alina S AS Khorolskaya Julia I JI Perepletchikova Daria A DA Blinova Miralda I MI Mikhailova Natalia A NA

International journal of molecular sciences 20240807 16

The functioning of the human cornea heavily relies on the maintenance of its extracellular matrix (ECM) mechanical properties. Within this context, corneal stromal fibroblasts (CSFs) are essential, as they are responsible for remodeling the corneal ECM. In this study, we used a decellularized human amniotic membrane (dHAM) and a custom fibrillar collagen film (FCF) to explore the effects of fibrillar materials on human CSFs. Our findings indicate that substrates like FCF can enhance the early de ...[more]

PMID: 39201288

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