Proteomics

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Label-Free Quantification Method for Assessing Sex from Modern and Ancient Bovine Tooth Enamel PART II

ABSTRACT: Identification of the sex of fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of fossils is often impossible. To overcome this, we have developed an innovative protocol to determine animal’s sex applying label-free quantification (LFQ) of two unique AmelY peptides ‘LRYPYP’ (AmelY;[M+2]2+ 404.7212 m/z) and ‘LRYPYPSY’ (AmelY;[M+2]2+ 529.7689 m/z) that are solely present in males that permitted determination of the animal’s sex. We examine the application of this method to eight (8) modern cattle (Bos taurus) and (12) twelve fossil (Bos) samples from a Neolithic sites of Beisamoun in Israel (8th–7th millennium BC). Our methodology correctly determinate sex in all modern and ancient samples.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Enamel, Tooth Enamel

DISEASE(S): Disease Free

SUBMITTER: Paula Kotli  

LAB HEAD: Elisabetta Boaretto

PROVIDER: PXD053148 | Pride | 2024-10-17

REPOSITORIES: Pride

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Source:
Action DRS
HF2_EB_20701_Control1_07032024.raw Raw
HF2_EB_20701_Control1_07032024.raw_20240317.mgf Mgf
HF2_EB_20701_Control2_07032024.raw Raw
HF2_EB_20701_Control2_07032024.raw_20240317.mgf Mgf
HF2_EB_20701_WIS101_07032024.raw Raw
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Publications

A label-free quantification method for assessing sex from modern and ancient bovine tooth enamel.

Kotli Paula P   Morgenstern David D   Bocquentin Fanny F   Khalaily Hamoudi H   Horwitz Liora Kolska LK   Boaretto Elisabetta E  

Scientific reports 20240806 1

Identification of the sex of modern, fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to many-factors, sex determination of osteological remains is often problematic. To overcome this, we have developed an innovative protocol to determine an animal's sex from tooth enamel, by applying label-free quantification (LFQ) of two unique AmelY peptides 'LRYPYP' (AmelY;[M+2] 2+ 404.7212 m/z) and 'LRYPYPSY' (Am  ...[more]

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