Proteomics

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Label-Free Quantification Method for Assessing Sex from Modern and Ancient Bovine Tooth Enamel. PART III

ABSTRACT: Identification of the sex of modern, fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of such remains is often impossible. To overcome this, we have developed an innovative protocol to determine an animal sex applying label-free quantification (LFQ) of two unique AmelY peptides ‘LRYPYP’ (AmelY;[M+2]2+ 404.7212 m/z) and ‘LRYPYPSY’ (AmelY;[M+2]2+ 529.7689 m/z) that are solely present in tooth enamel of males. we applied this method to eight (8) modern cattle (Bos taurus) and (12) twelve archaeological samples of Bos from a Neolithic sites of Beisamoun, Israel (8th–7th millennium BC). Our methodology correctly identified the sex of the modern samples and enabled determination of sex in the archaeological samples.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Tooth Enamel, Tooth Enamel Organ

DISEASE(S): Disease Free

SUBMITTER: Paula Kotli  

LAB HEAD: Prof. Elisabetta Boaretto

PROVIDER: PXD053225 | Pride | 2024-10-17

REPOSITORIES: Pride

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HF2_EB_20701_WIS100_07032024.raw Raw
HF2_EB_20701_WIS100_07032024.raw_20240317.mgf Mgf
HF2_EB_20701_WIS100_07032024.raw_20240317.mzid Mzid
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HF2_EB_20701_WIS101_07032024.raw Raw
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Publications

A label-free quantification method for assessing sex from modern and ancient bovine tooth enamel.

Kotli Paula P   Morgenstern David D   Bocquentin Fanny F   Khalaily Hamoudi H   Horwitz Liora Kolska LK   Boaretto Elisabetta E  

Scientific reports 20240806 1

Identification of the sex of modern, fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to many-factors, sex determination of osteological remains is often problematic. To overcome this, we have developed an innovative protocol to determine an animal's sex from tooth enamel, by applying label-free quantification (LFQ) of two unique AmelY peptides 'LRYPYP' (AmelY;[M+2] 2+ 404.7212 m/z) and 'LRYPYPSY' (Am  ...[more]

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